کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11030379 | 1646317 | 2018 | 18 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Modular organization analysis of specific naringin/neoeriocitrin related gene expression induced by UVC irradiation in Drynaria roosii
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کلمات کلیدی
HCTNNCKEGGUVC irradiationp-coumarate 3-hydroxylase4-Coumarate CoA ligase4CLWGCNAFPKMCNRNNSbHLHC3HCORFDRNGSCHICHSPALRNA-seqC4Hbasic helix-loop-helix - اسلحه پایه حلقه ایWeighted gene co-expression network analysis - تجزیه و تحلیل شبکه همبستگی ژن با وزنRNA sequencing - ترتیب RNANext-generation sequencing - تعیین توالی نسل بعدیKyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوchalcone synthase - سنتاز چالکونcinnamate 4-hydroxylase - سینامات 4-هیدروکسیلازDEG - شماMass spectroscopy - طیف سنجی جرمیTranscription factor - عامل رونویسیPhenylalanine ammonia lyase - فنیل آلانین آمونیاک لیائازfalse discovery rate - میزان کشف کاذبGene ontology - هستیشناسی ژنیhydroxycinnamoyl transferase - هیدروکسی سینمویل ترانسفرازDifferentially expressed gene - ژن بیان شده متفاوت استchalcone isomerase - کلومون ایزومراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Drynaria roosii is a traditional medicinal fern, whose rhizome is particularly valued for its effective flavonoid components of naringin/neoeriocitrin. UV irradiation can induce flavonoid accumulation in plants, however the molecular mechanism of UVC-mediated naringin/neoeriocitrin synthesis in D. roosii has never been reported. Our HPLC-tandem mass spectrometry (MS/MS) results showed that UVC irradiation increased naringin/neoeriocitrin contents (NNCs) based on irradiation dosage in D. roosii. According to the NGS data, DEG-based heat map analysis revealed up-regulation of naringin/neoeriocitrin synthetic genes was more obvious in 12h UVC irradiation (UV dose = 81.648âkJâmâ2) when compared with 24h (UV dose = 163.296âkJâmâ2), especially for PAL, C3'H and HCT in old rhizomes. Through systems biology method of modular organization analysis, we clustered 15,678 DEGs into 19 modules, and calculated correlation coefficients between modules and samples, as well as modules and NNCs. Four significant naringin-related modules were achieved and displayed high correlations with specific samples. Moreover, weighted gene co-expression network analysis results discovered that 4CL, CHS and HCT, C3H acted as the hub responsive genes to UVC irradiation involved in naringin and neoeriocitrin synthesis respectively, and presented high co-expression with MYB/bHLH-regulated DEGs. Overall, we demonstrated that UVC irradiation up-regulated the naringin/neoeriocitrin related gene expression in separate new and old rhizomes to enhance NNCs in a dose-dependent manner. Hormetic UVC dose ranges improving NNCs in D. roosii were established for the first time. Our work also provided new insights into the study of secondary metabolites in medicinal plants.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental and Experimental Botany - Volume 156, December 2018, Pages 298-315
Journal: Environmental and Experimental Botany - Volume 156, December 2018, Pages 298-315
نویسندگان
Jing-Yi Li, Dong Li, Xue Du, Hui Li, Di Wang, Quan Xing, Ran Yao, Mei-Yu Sun, Lei Shi,