کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2014870 | 1541952 | 2013 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Overexpression of a chrysanthemum transcription factor gene, DgWRKY3, in tobacco enhances tolerance to salt stress
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کلمات کلیدی
GSHMDACaMVqRT-PCRAPXLEACATABAASAPODROS - ROSHydrogen peroxide - آب اکسیژنهAscorbate - آسکوربات یا ویتامین ثabscisic acid - اسید آبسزیکGene expression - بیان ژنrapid amplification of cDNA ends - تقویت سریع cDNA به پایان می رسدTransgenic tobacco - تنباکوی ترانس ژنSalt stress - تنش شوریLate embryogenesis abundant - زودرس زودرس فراوان استSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازWRKY transcription factor - فاکتور رونویسی WRKYmalondialdehyde - مالون دی آلدهیدRace - مسابقهwild type - نوع وحشیH2O2 - هیدروژن پراکسیدquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استPeroxidase - پراکسیدازascorbate peroxidase - پراکسیداز آسکورباتیCatalase - کاتالازChrysanthemum - گل داودیGlutathione - گلوتاتیونReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
WRKY transcription factor genes (TFs) play important roles in response to various abiotic stresses. However, the roles of the chrysanthemum WRKY genes in abiotic stress response remain obscure. In this study, we functionally characterized a novel WRKY gene, DgWRKY3, from chrysanthemum (Dendranthema grandiflorum). Its expression in the chrysanthemum was up-regulated by salinity or dehydration stress, but not by abscisic acid (ABA). The DgWRKY3-overexpression tobacco plants increase salt tolerance compared with wild-type (WT) tobacco plants. The increased levels of proline were observed in transgenic plants compared to WT plants under salt stress. In addition, the DgWRKY3 transgenic plants reduced accumulation of malondialdehyde (MDA) and hydrogen peroxide (H2O2) compared with WT plants, accompanied by higher activities of antioxidant enzymes including superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) and the greater accumulation of antioxidants including ascorbate (AsA) and glutathione (GSH) under salt stress. Moreover, the DgWRKY3 transgenic plants enhanced the expression of stress-related genes involved in osmotic adjustment and membrane protection (NtP5CS, NtLEA5, and NtERD10D) and oxidative stress response (NtSOD, NtPOD, NtCAT, and NtAPX) under salt stress. However, no significant difference in the expression of stress-related genes (NtP5CS, NtLEA5, NtERD10D, NtSOD, NtPOD, NtCAT, and NtAPX) was found between the DgWRKY3-overexpression and WT tobacco plants under normal conditions, despite the fact that the constitutive promoter was used to drive DgWRKY3. These findings suggest that DgWRKY3 functions as a positive regulator to mediate tolerance of plants to salt stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 69, August 2013, Pages 27-33
Journal: Plant Physiology and Biochemistry - Volume 69, August 2013, Pages 27-33
نویسندگان
Qing-Lin Liu, Ming Zhong, Shuang Li, Yuan-Zhi Pan, Bei-Bei Jiang, Yin Jia, Hai-Qing Zhang,