کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016560 | 1541974 | 2009 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Overexpression of endoplasmic reticulum omega-3 fatty acid desaturase gene improves chilling tolerance in tomato
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کلمات کلیدی
PVDFSQDGPFDDASDGDGmonogalactosyldiacylglycerolMGDG18:1 - 18: 118:2 - 18: 218:3 - 18: 3TAS - IASOleic acid - اسید اولئیکLinoleic acid - اسید لینولئیکLinolenic acid - اسید لینولینیکSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتGene expression - بیان ژنChilling tolerance - تحمل دلسردیphoton flux density - تراکم شار فوتونendoplasmic reticulum - شبکه آندوپلاسمی phosphatidylcholine - فسفاتیدیل کولینphosphatidylglycerol - فسفاتیدیل گلیسرولPolyvinylidene fluoride - پلی وینیلیدین فلورایدTomato (Lycopersicon esculentum) - گوجه فرنگی (Lycopersicon esculentum)
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Overexpression of endoplasmic reticulum omega-3 fatty acid desaturase gene improves chilling tolerance in tomato Overexpression of endoplasmic reticulum omega-3 fatty acid desaturase gene improves chilling tolerance in tomato](/preview/png/2016560.png)
چکیده انگلیسی
An endoplasmic reticulum-localized tomato omega-3 fatty acid desaturase gene (LeFAD3) was isolated and characterized with regard to its sequence, response to various temperatures and function in transgenic tomato plants. Northern blot analysis showed that LeFAD3 was expressed in all organs tested and was markedly abundant in roots. Meanwhile, the expression of LeFAD3 was induced by chilling stress (4 °C), but inhibited by high temperature (40 °C). The transgenic plants were obtained under the control of the cauliflower mosaic virus 35S promoter (35S-CaMV). Northern and western blot analyses confirmed that sense LeFAD3 was transferred into tomato genome and overexpressed. Level of linolenic acids (18:3) increased and correspondingly level of linoleic acid (18:2) decreased in leaves and roots. After chilling stress, the fresh weight of the aerial parts of transgenic plants was higher than that of the wild type (WT) plants, and the membrane system ultrastructure of chloroplast in leaf cell and all the subcellular organelles in root tips of transgenic plants kept more intact than those of WT. Relative electric conductivity increased less in transgenic plants than that in WT, and the respiration rate of the transgenic plants was notably higher than that of WT. The maximal photochemical efficiency of PSII (Fv/Fm) and the O2 evolution rate in WT decreased more than those in transgenic plants under chilling stress. Together with other data, results showed that the overexpression of LeFAD3 led to increased level of 18:3 and alleviated the injuries under chilling stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 47, Issues 11â12, NovemberâDecember 2009, Pages 1102-1112
Journal: Plant Physiology and Biochemistry - Volume 47, Issues 11â12, NovemberâDecember 2009, Pages 1102-1112
نویسندگان
Chao Yu, Hua-Sen Wang, Sha Yang, Xian-Feng Tang, Ming Duan, Qing-Wei Meng,