کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2016770 | 1067687 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Metabolic footprinting study of white spruce somatic embryogenesis using NMR spectroscopy
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کلمات کلیدی
ABAN6-benzyladeninePLSBCKAnuclear magnetic resonance - رزونانس مغناطیسی هستهای2,4-D - 2،4-D2,4-dichlorophenoxyacetic acid - 2،4-dichlorophenoxyacetic اسیدPCA - PCAbranched chain amino acids - آمینو اسیدهای زنجیرهای منشعب شدهγ-aminobutyric acid - اسید γ-آمینوبوتیریکabscisic acid - اسید آبسزیکMaturation - بلوغBCAA - بی سی ای ایPrincipal component analysis - تحلیل مولفههای اصلی یا PCANMR - تشدید مغناطیسی هستهای Embryogenesis - جنین زاییSomatic embryo - جنین سمیPartial least squares - حداقل مربعات جزئی White spruce - صنوبر سفیدNMR spectroscopy - طیف سنجی رزونانس مغناطیسی هستهMaintenance - عملیات نگهداری و تعمیراتMetabolomics - متابولومیکChemometrics - کمومتریکسGABA - گاباOne dimensional - یک بعدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
White spruce is an important commercial species for reforestation. The success in its propagation through somatic embryogenesis is well documented; however the physiological processes involved are poorly understood and remain unoptimized. The variable quality embryos generated in vitro from the same genotype suggest control at the protein and metabolite level. In order to probe metabolic changes, we have conducted a “metabolic footprinting” study, whereby culture media from growing cells was quantitatively analyzed to determine which metabolites were consumed and excreted. Such experiments are advantageous in that there is no need to quench cellular metabolism or extract intracellular metabolites through time-consuming protocols. In this paper we demonstrate the application of the footprinting assay to somatic embryo cells of white spruce (Picea glauca) using 1D 1H NMR spectroscopy. We have surveyed embryogenesis metabolism in two types of media, maintenance (MN) and maturation (MT). MN medium does not result in shoot apical meristem (SAM) formation, while MT medium induces the necessary changes leading to fully developed somatic embryos. The two types of media were easily distinguished using metabolomics analysis, namely multivariate pattern recognition statistics (orthogonal partial least squares discriminatory analysis). From this analysis, we have identified numerous compounds involved with branched chain amino acid pathways such as valine and isoleucine. These results are explained on the basis of known metabolic pathways implicated in plant and animal developmental processes, and ultimately implicate altered CoA biosynthesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Plant Physiology and Biochemistry - Volume 47, Issue 5, May 2009, Pages 343-350
Journal: Plant Physiology and Biochemistry - Volume 47, Issue 5, May 2009, Pages 343-350
نویسندگان
Reza Dowlatabadi, Aalim M. Weljie, Trevor A. Thorpe, Edward C. Yeung, Hans J. Vogel,