کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2130056 | 1086525 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adrenomedullin attenuates interleukin-1β-induced inflammation and apoptosis in rat Leydig cells via inhibition of NF-κB signaling pathway
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کلمات کلیدی
cyclo-oxygenase-2PGE2iNOSTBARSDAPICOX-2GSHIL-1βRIAADMPARPFBSDMEMPBSH2DCF-DAMDANF-κB2′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetateDulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده DulbeccoMTT - MTTROS - ROSadrenomedullin - آدرنومدولینinflammation - التهاب( توروم) Interleukin-1β - اینترلوکین-1βterminal deoxynucleotidyl transferase dUTP nick-end labeling - ترمینال deoxynucleotidyl transferase dUTP نهایی پایان برچسبELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاTUNEL - تونلApoptosis - خزان یاختهایradioimmunoassay - رادیوایمونواسیfetal bovine serum - سرم جنین گاوLeydig cell - سلول لیدینگinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییNuclear factor-kappa B - فاکتور هسته ای-کاپا Bmalondialdehyde - مالون دی آلدهیدphosphate buffer solution - محلول بافر فسفاتthiobarbituric acid reactive substances - مواد واکنش پذیر اسید تیوباربیتوریکNitric oxide - نیتریک اکسیدpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازProstaglandin E2 - پروستاگلاندین E2Glutathione - گلوتاتیونReactive oxidative species - گونه های واکنش پذیر اکسیداتیو
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The aim of this paper is to investigate the protective effects of adrenomedullin (ADM) on interleukin-1β (IL-1β)-induced inflammation and apoptosis in rat Leydig cells and its underlying molecular mechanisms. Leydig cells were isolated from adult Sprague-Dawley rats. The cell culture was established by adding ADM 2 h prior to 24 h treatment with IL-1β-induced cytotoxicity. We detected cell viability and concentrations of testosterone, reactive oxygen species (ROS), malondialdehyde (MDA), and reduced glutathione (GSH). Gene expression levels were measured for inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2). Concentrations were detected for nitric oxide (NO) and prostaglandin E2 (PGE2). Apoptosis was assessed using terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL). Levels of gene expression and protein were detected for Bcl-2, Bax, caspase-3, and poly adenosine diphosphate-ribose polymerase (PARP). Protein levels were measured for nuclear factor kappa B (NF-κB) p65 and IκBα. ADM reduced IL-1β-induced cytotoxicity. ADM pretreatment significantly increased testosterone concentrations and decreased ROS, MDA, and GSH concentrations. ADM pretreatment inhibited IL-1β-induced inflammation in Leydig cells by decreasing the gene expression levels of iNOS and COX-2, as well as the concentrations of NO and PGE2. ADM pretreatment further decreased the number of TUNEL-positive stained Leydig cells, as confirmed by the increase in gene expression and protein levels of Bcl-2 and the decrease of Bax, caspase-3, and PARP levels. Moreover, ADM pretreatment inhibited NF-κB p65 phosphorylation and IκBα phosphorylation and degradation. ADM has potential anti-inflammatory and anti-apoptotic properties in IL-1β-induced rat Leydig cells, which might be related to NF-κB signaling pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 339, Issue 2, 10 December 2015, Pages 220-230
Journal: Experimental Cell Research - Volume 339, Issue 2, 10 December 2015, Pages 220-230
نویسندگان
Wei Hu, Pang-hu Zhou, Ting Rao, Xiao-bin Zhang, Wei Wang, Li-jun Zhang,