کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2603992 | 1133858 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro responses of rat alveolar macrophages to particle suspensions and water-soluble components of dust storm PM2.5
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کلمات کلیدی
8-anilino-1-naphthalene-sulfonic acidTBARSLPOGSHDTNBEGTARNSHEPESDPHFBSRat alveolar macrophages1,6-diphenyl-1,3,5-hexatriene - 1،6-دیفنیل-1،3،5-هگزیدریلFURA-2/AM - 2-فورا / AM3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromide5,5′-dithio-bis(2-nitrobenzoic acid) - 5،5'-dithio-bis (2-nitrobenzoic acid)BSA - BSADMSO - DMSOMTT - MTTN-2-hydroxy-ethylpiperazine-N′-2-ethanesulfonic acid - N-2-هیدروکسی اتیل پپپرازین-N'-2-ethanesulfonic acidROS - ROSadenosinetriphosphatase - آدنوزینتری فسفاتازbovine serum albumin - آلبومین سرم گاوOxidant stress - استرس اکسیدانATPase - ایتیپیایزها Dimethyl sulfoxide - دیمتیل سولفواکسیدFine particulate matter - ذرات جامدCell viability - زنده ماندن سلولANS - سالfetal bovine serum - سرم جنین گاوDust storm - طوفان گرد و غبارlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Alveolar macrophages - ماکروفاژهای آلوئولارLipid peroxidation - پراکسیداسیون لیپیدGlutathione - گلوتاتیونthiobarbituric acid-reactive species - گونه های واکنش پذیر اسید تیوباربیتوریکreactive nitrogen species - گونه های واکنش پذیر نیتروژنReactive oxygen species - گونههای فعال اکسیژنCalcium ion - یون کلسیم
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A study was conducted to investigate the in vitro toxicities of dust storm particulate matter with an aerodynamic diameter ⩽ 2.5 μm (PM2.5) on rat alveolar macrophages (AM). This was based on the ambient PM2.5 collected in March 2004 from Baotou city, Inner Mongolia Autonomous Region, China. The particles were classified as normal (from sunshiny and clean days) and dust storm samples according to the dust storm classification, and the local weather and air quality monitoring data. The cell viability, levels of cellular thiobarbituric acid-reactive species (TBARS), glutathione (GSH), and cytosolic free calcium ions (Ca2+), and the plasma membrane ATPase activities and membrane lipid fluidity were determined 4 h following the in vitro treatment of AM with differing dosages of the collected samples. Results revealed that dust storm PM2.5 and their water-soluble fractions at high dosages generated oxidant stress on AM, induced leakage of lactate dehydrogenase (LDH), significantly decreased activities of plasma membrane Na+K+-ATPase, increased intracellular Ca2+ levels, and led to significant alterations in membrane lipid fluidity, finally resulting in cytotoxicity. A two-way ANOVA showed that there was no significant difference in the above indices measured between the normal and dust storm PM2.5, suggesting the deleterious effects of ambient PM2.5 from Baotou city were based on the dose used rather than the type of particles. But due to a higher concentration of airborne PM2.5 mass concentration in dust storm days than that in normal days, it concluded that during dust storm episodes, a much more serious effect on AM was imminent.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 20, Issue 5, August 2006, Pages 575-584
Journal: Toxicology in Vitro - Volume 20, Issue 5, August 2006, Pages 575-584
نویسندگان
Hong Geng, Ziqiang Meng, Quanxi Zhang,