کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
4754353 | 1418059 | 2017 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The photodynamic action of pheophorbide a induces cell death through oxidative stress in Leishmania amazonensis
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کلمات کلیدی
FBSPDTNACIC50DTNBRNSN-acetyl-l-cysteineLeishmania amazonensisMDCΔΨmCCCPtetramethylrhodamineantimycin ATMREDPPPPheophorbide aLPSRPMI-1640DAF-FM diacetatePBSIC90WTM2′,7′-dichlorofluorescin diacetate - 2 '، 7'-dichlorofluorescin diacetateDMSO - DMSOH2DCFDA - H2DCFD بهMTT - MTTO2− - O2-ROS - ROSHydrogen peroxide - آب اکسیژنه superoxide anion - آنیون سوپر اکسیدTem - این استOxidative stress - تنش اکسیداتیوPhotodynamic therapy - درمان فتودینامیکRoom temperature - دمای اتاقdiphenyl-1-pyrenylphosphine - دیفنیل-1-پیرینیل فسفینDimethylsulfoxide - دیمتیل سولفواکسیدfetal bovine serum - سرم جنین گاوPhosphatidylserine - فسفاتیدیلسرینlipopolysaccharide - لیپوپلی ساکاریدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریCell death - مرگ سلولی Roswell Park Memorial Institute medium - موسسه خاطرات Roswell Park mediumMonodansylcadaverine - مونودانسیل کاداورینTransmission electron microscopy - میکروسکوپ الکترونی عبوریNitric oxide - نیتریک اکسیدH2O2 - هیدروژن پراکسیدwortmannin - ورتمنینMitochondrial membrane potential - پتانسیل غشای میتوکندریPropidium iodide - پروتئین یدیدreactive nitrogen species - گونه های واکنش پذیر نیتروژنReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Leishmaniasis is a disease caused by hemoflagellate protozoa, affecting millions of people worldwide. The difficulties of treating patients with this parasitosis include the limited efficacy and many side effects of the currently available drugs. Therefore, the search for new compounds with leishmanicidal action is necessary. Photodynamic therapy has been studied in the medical field because of its selectivity, utilizing a combination of visible light, a photosensitizer compound, and singlet oxygen to reach the area of treatment. The continued search for selective alternative treatments and effective targets that impact the parasite and not the host are fundamentally important for the development of new drugs. Pheophorbide a is a photosensitizer that may be promising for the treatment of leishmaniasis. The present study evaluated the in vitro biological effects of pheophorbide a and its possible mechanisms of action in causing cell death in L. amazonensis. Pheophorbide a was active against promastigote and amastigote forms of the parasite. After treatment, we observed ultrastructural alterations in this protozoan. We also observed changes in promastigote macromolecules and organelles, such as loss of mitochondrial membrane potential [â Ψm], lipid peroxidation, an increase in lipid droplets, DNA fragmentation, phosphatidylserine exposure, an increase in caspase-like activity, oxidative imbalance, and a decrease in antioxidant defense systems. These findings suggest that cell death occurred through apoptosis. The mechanism of cell death in intracellular amastigotes appeared to involve autophagy, in which we clearly observed an increase in reactive oxygen species, a compromised â Ψm, and an increase in the number of autophagic vacuoles. The present study contributes to the development of new photosensitizers against L. amazonensis. We also elucidated the mechanism of action of pheophorbide a, mainly in intracellular amastigotes, which is the most clinically relevant form of this parasite.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 174, September 2017, Pages 342-354
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 174, September 2017, Pages 342-354
نویسندگان
Nathielle Miranda, Hélito Volpato, Jean Henrique da Silva Rodrigues, Wilker Caetano, Tânia Ueda-Nakamura, Sueli de Oliveira Silva, Celso Vataru Nakamura,