کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5507690 | 1400342 | 2017 | 25 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Histone methyltransferase SETDB1 maintains survival of mouse spermatogonial stem/progenitor cells via PTEN/AKT/FOXO1 pathway
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کلمات کلیدی
SFKembryo stem cellshistone H3 lysine 9SETDB1APAF1H3K9SSCsXIAPDNA methyltransferasesPRC2ESCsDNMTsGDNFPI3KFBSBiFCHEKDMEM/F12 - DMEM / F12Src family kinase - kinase family SrcAkt - آکتchromatin immunoprecipitation - ایمن سازی کروماتینbimolecular fluorescence complementation - تکمیل فلورسانس دو طرفه حرکتیApoptosis - خزان یاختهایfetal bovine serum - سرم جنین گاوSpermatogonial stem cells - سلول های بنیادی اسپرماتوگونالیapoptotic protease activating factor 1 - عامل فعال آپوپتوز پروتئاز 1Glial cell line-derived neurotrophic factor - فاکتور نوروتروفی مشتق از سلول گلیاییPhosphatidylinositol 3-kinase - فسفاتیدیلینواستیل 3-کینازMethylation - متیلاسیونX-linked Inhibitor of Apoptosis Protein - پروتئین آپوپتوز وابسته به X-linkedpolycomb repressive complex 2 - پیچک سرکوبگر پلی کامب 2CHiP - چیپhuman embryonic kidney - کلیه جنین انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Spermatogonial stem cells (SSCs) possess the capacity of self-renewal and differentiation, which are the basis of spermatogenesis. In maintenance of SSC homeostasis, intrinsic/extrinsic factors and various signaling pathways tightly control the fate of SSCs. Methyltransferase SETDB1 (Set domain, bifurcated 1) catalyzes histone H3 lysine 9 (H3K9) trimethylation and represses gene expression. SETDB1 is required for maintaining the survival of spermatogonial stem cells in mice. However, the underlying molecular mechanism remains unclear. In the present study, we found that Setdb1 regulates PTEN/AKT/FOXO1 pathway to inhibit SSC apoptosis. Co-immunoprecipitation and reporter gene assay revealed that SETDB1 interacted and coordinated with AKT to regulate FOXO1 activity and expression of the downstream target genes Bim and Puma. Among the SETDB1-bound genes, the H3K9me3 levels on the promoter regions of Bim and Pten decreased in Setdb1-KD group; in contrast, H3K9me3 status on promoters of Bax and Puma remained unchanged. Therefore, SETDB1 was responsible for regulating the transcription activity of genes in the apoptotic pathway at least in part through modulating H3K9me3. This study replenishes the research on the epigenetic regulation of SSC survival, and provides a new insight for the future study of epigenetic regulation of spermatogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1860, Issue 10, October 2017, Pages 1094-1102
Journal: Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms - Volume 1860, Issue 10, October 2017, Pages 1094-1102
نویسندگان
Tiantian Liu, Xiaoxu Chen, Tianjiao Li, Xueliang Li, Yinghua Lyu, Xiaoteng Fan, Pengfei Zhang, Wenxian Zeng,