کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5745901 | 1618782 | 2017 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
RNA sequencing indicates that atrazine induces multiple detoxification genes in Daphnia magna and this is a potential source of its mixture interactions with other chemicals
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کلمات کلیدی
BPAuridine 5′-diphospho-glucuronosyltransferaseUDPGTPXRMRPADHGSTCyPqPCRRNAseqABC transporter - ABC حمل کنندهAUC - AUCROS - ROSdocosahexaenoic acid - اسید داکوزاگزوائونیکInduction - القاءAlcohol dehydrogenase - الکل دهیدروژنازGene expression - بیان ژنBisphenol A - بیسفنول ای، بیسفنول ARNA sequencing - ترتیب RNAATP-binding cassette transporter - حمل کننده کاسه اتصال ATPKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوDHA - دوکوساهگزائنوئیک اسیدAquatic toxicology - سم شناسی آبزیCytochrome P450 - سیتوکروم پی۴۵۰CAR - ماشینMixture model - مدل مخلوطarea under the curve - منطقه تحت منحنیGene ontology - هستیشناسی ژنیquantitative real-time polymerase chain reaction - واکنش زنجیره ای پلیمراز کمی زمان واقعی استMulti-drug resistance protein - پروتئین مقاوم در برابر چند داروglutathione S-transferase - گلوتاتیون S-ترانسفرازReactive oxygen species - گونههای فعال اکسیژنconstitutive androstane receptor - گیرنده آندروستان پایدارPregnane X receptor - گیرنده پیش گران XNuclear receptor - گیرنده هستهای، گیرندههای هستهای
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
شیمی زیست محیطی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Atrazine is an herbicide with several known toxicologically relevant effects, including interactions with other chemicals. Atrazine increases the toxicity of several organophosphates and has been shown to reduce the toxicity of triclosan to D. magna in a concentration dependent manner. Atrazine is a potent activator in vitro of the xenobiotic-sensing nuclear receptor, HR96, related to vertebrate constitutive androstane receptor (CAR) and pregnane X-receptor (PXR). RNA sequencing (RNAseq) was performed to determine if atrazine is inducing phase I-III detoxification enzymes in vivo, and estimate its potential for mixture interactions. RNAseq analysis demonstrates induction of glutathione S-transferases (GSTs), cytochrome P450s (CYPs), glucosyltransferases (UDPGTs), and xenobiotic transporters, of which several are verified by qPCR. Pathway analysis demonstrates changes in drug, glutathione, and sphingolipid metabolism, indicative of HR96 activation. Based on our RNAseq data, we hypothesized as to which environmentally relevant chemicals may show altered toxicity with co-exposure to atrazine. Acute toxicity tests were performed to determine individual LC50 and Hillslope values as were toxicity tests with binary mixtures containing atrazine. The observed mixture toxicity was compared with modeled mixture toxicity using the Computational Approach to the Toxicity Assessment of Mixtures (CATAM) to assess whether atrazine is exerting antagonism, additivity, or synergistic toxicity in accordance with our hypothesis. Atrazine-triclosan mixtures showed decreased toxicity as expected; atrazine-parathion, atrazine-endosulfan, and to a lesser extent atrazine-p-nonylphenol mixtures showed increased toxicity. In summary, exposure to atrazine activates HR96, and induces phase I-III detoxification genes that are likely responsible for mixture interactions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemosphere - Volume 189, December 2017, Pages 699-708
Journal: Chemosphere - Volume 189, December 2017, Pages 699-708
نویسندگان
Allison M. Schmidt, Namrata Sengupta, Christopher A. Saski, Rooksana E. Noorai, William S. Baldwin,