کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5847831 | 1561602 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protective role of naringin against cisplatin induced oxidative stress, inflammatory response and apoptosis in rat striatum via suppressing ROS-mediated NF-κB and P53 signaling pathways
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کلمات کلیدی
DCHF-DATNFαqPCRTBARSPCOCATMDATBADNPHGPX2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetateNFκB - NFKBROS - ROSStriatum - استریاتومThiobarbituric acid - اسید تیوباربیتوریکinflammation - التهاب( توروم) P53 expression - بیان P53Oxidative stress - تنش اکسیداتیوtumor necrosis factor-α - تومور نکروز عامل αSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازChemotherapy - شیمیدرمانیnuclear factor κB - فاکتور هسته ای κBmalondialdehyde - مالون دی آلدهیدthiobarbituric acid reactive substances - مواد واکنش پذیر اسید تیوباربیتوریکAged rat - موش سالمquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیProtein carbonyls - پروتئین کربونیلCatalase - کاتالازglutathione peroxidase - گلوتاتیون پراکسیدازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
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چکیده انگلیسی
Cisplatin (Cis) is an effective chemotherapeutic agent successfully used in the treatment of a wide range of malignancies while its usage is limited due to its dose-dependent toxicity. The present study was conducted to investigate the efficacy of naringin, an ubiquitous flavonoid, against Cis-induced striatum injury in Wistar aged rats. Briefly, the experimental procedures were divided in two sets of experiments. In the first, the animals were divided into 4 groups: control, Nar 25 mg/kg, Nar 50 mg/kg and Nar 100 mg/kg. In the second, the animals were divided into 4 groups: Cis (5 mg/kg/week for 5 consecutive weeks), Cis + Nar (25 mg/kg), Cis + Nar (50 mg/kg) and Cis + Nar (100 mg/kg). The administration of Cis (5 mg/kg/week for 5 consecutive weeks) resulted in a decline in the concentrations of reduced glutathione and ascorbic acid. The activity of membrane bound ATPases and glutathione peroxidase (GPx) were decreased while the activity of catalase (CAT) and superoxide dismutase (SOD) were increased. Further, in striatum tissue, Cis significantly enhance the mRNA gene expression of P53, nuclear factor κB pathway (NFκB) and tumor necrosis factor (TNF-α). Oxidative/nitrosative stress was evident in Cis group by increased malondialdehyde (MDA), protein carbonyls (PCO), reactive oxygen species (ROS) and nitrite concentration (NO). Naringin (25, 50 and 100 mg/kg) administration was able to protect against deterioration in striatum tissue, abrogate the change in antioxidant enzyme activities and suppressed the increase in MDA, PCO, NO and TNF-α concentrations. Moreover, Nar inhibited P53, NFkB and TNF-α pathways mediated inflammation and apoptosis, and improved the histological changes induced by Cis. Thus, these findings demonstrated the neuroprotective nature of Nar by attenuating the pro-inflammatory and apoptotic mediators and improving antioxidant competence in striatum tissue. These results imply that Nar has perfect effect against Cis-induced striatum injury in aged rats, which should be developed as an effective food and healthcare product for the treatment of brain injury in the future.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 239, 5 September 2015, Pages 76-86
Journal: Chemico-Biological Interactions - Volume 239, 5 September 2015, Pages 76-86
نویسندگان
Yassine Chtourou, Bakhta Aouey, Mohammed Kebieche, Hamadi Fetoui,