Influence of tamoxifen on gluconeogenesis and glycolysis in the perfused rat liver

► We investigated how tamoxifen acts on liver metabolism. ► The action of tamoxifen on hepatic gluconeogenesis and glycolysis was measured. ► Tamoxifen stimulates glycolysis and inhibits gluconeogenesis. ► The mechanism of the action of tamoxifen is complex. ► Glucose homeostasis in the presence of tamoxifen requires regulatory adaptations.