کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5861292 | 1133757 | 2015 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Expression of xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
TEERphysiologically based pharmacokinetic modellingRPTEC/TERT1RFUOATPABCPBPKepithelia - اپیتلیاOct - اکتبرCSA - ایالات مؤتلفهٔ آمریکاOat - جو دو سرorganic cation transporter - حمل و نقل کاتیونی آلیTransport - حمل و نقل یا ترابریorganic anion transporter - حمل کننده آنیون آلیMate - رفیقCyclosporine A - سیکلوسپورینAtransepithelial electrical resistance - مقاومت الکتریکی transepithelial
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Expression of xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1 Expression of xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1](/preview/png/5861292.png)
چکیده انگلیسی
The kidney is a major target for drug-induced injury, primarily due the fact that it transports a wide variety of chemical entities into and out of the tubular lumen. Here, we investigated the expression of the main xenobiotic transporters in the human renal proximal tubule cell line RPTEC/TERT1 at an mRNA and/or protein level. RPTEC/TERT1 cells expressed OCT2, OCT3, OCTN2, MATE1, MATE2, OAT1, OAT3 and OAT4. The functionality of the OCTs was demonstrated by directional transport of the fluorescent dye 4-Di-1-ASP. In addition, P-glycoprotein activity in RPTEC/TERT1 cells was verified by fluorescent dye retention in presence of various P-glycoprotein inhibitors. In comparison to proliferating cells, contact inhibited RPTEC/TERT1 cells expressed increased mRNA levels of several ABC transporter family members and were less sensitive to cyclosporine A. We conclude that differentiated RPTEC/TERT1 cells are well suited for utilisation in xenobiotic transport and pharmacokinetic studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 30, Issue 1, Part A, 25 December 2015, Pages 95-105
Journal: Toxicology in Vitro - Volume 30, Issue 1, Part A, 25 December 2015, Pages 95-105
نویسندگان
Lydia Aschauer, Giada Carta, Nadine Vogelsang, Eberhard Schlatter, Paul Jennings,