کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5904591 | 1158014 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dietary supplementation of milk fermented with probiotic Lactobacillus fermentum enhances systemic immune response and antioxidant capacity in aging mice
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کلمات کلیدی
RBCMCP-1MRSDCsNDRIPBSGPXIL-4CATMPO - DFOIgG1 - IgG1 وAntioxidant - آنتی اکسیدانEscherichia coli - اشریشیا کُلیInflammaging - التهابImmunosenescence - ایمن سازیImmunoglobulin G1 - ایمونوگلوبولین G1Interleukin 4 - اینترلوکین 4analysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاtumor necrosis factor α - تومور نکروز عامل αbasal diet - رژیم غذایی پایهSOD - سدDendritic cells - سلول های دندریتیکSuperoxide dismutase - سوکسوکس دیسموتازSkim milk - شیر خشکInfection - عفونتTNF-α - فاکتور نکروز توموری آلفاLactobacillus - لاکتوباسیلوسPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریMice - موشmyeloperoxidase - میلوپراکسیداز E coli - و کولیmonocyte chemotactic protein 1 - پروتئین chemotactic monocyte 1Catalase - کاتالازred blood cell - گلبول قرمز، اریتروسیتglutathione peroxidase - گلوتاتیون پراکسیداز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Although probiotics are known to enhance the host immune response, their roles in modulating immunosenescence, resisting infection, and improving redox homeostasis during aging remain unclear. Therefore, the present study was devised in aging mice to assess the antiimmunosenescence potential from the consumption of milk that is fermented with probiotic Lactobacillus fermentum MTCC 5898 (LF). We hypothesized that probiotic supplementation would boost immunity, improve antioxidant capacity, and resist severity of pathogenic infection in aging mice. To test this hypothesis, during a trial period of 2 months, 16-month-old male Swiss mice were kept on 3 experimental diets: basal diet (BD), BD supplemented with skim milk, and BD supplemented with probiotic LF-fermented milk. A concurrent analysis of several immunosenescence markers that include neutrophil functions, interleukins profile, inflammation and antibody responses in the intestine as well as analysis of antioxidant enzymes in the liver and red blood cells was performed. Neutrophil respiratory burst enzymes and phagocytosis increased significantly in probiotic LF-fed groups, whereas no exacerbation in plasma levels of monocyte chemotactic protein 1 and tumor necrosis factor α was observed. Splenocytes registered increased interferon-γ but decreased interleukin 4 and interleukin 10 production, whereas humoral antibodies registered decreases in immunoglobulin G1 (IgG1)/IgG2a ratio and IgE levels in the probiotic-fed groups. Antioxidant enzymes (superoxide dismutase, catalase, and glutathione peroxidase) in LF-fed groups showed increased activities, which were more pronounced in the liver than in red blood cell. An Escherichia coli-based infection model in aging mice was also designed to validate the protective attributes of LF. Administration of probiotic LF significantly reduced E coli population in organs (intestine, liver, spleen, and peritoneal fluid), as compared with control groups, by enhancing E coli-specific antibodies and inflammatory proteins. Based on these results, it appears that LF supplementation alleviated immunosenescence, enhanced antioxidant enzyme activities, and resisted E coli infection in aging mice; thereby, signifying its potential in augmenting healthy aging.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nutrition Research - Volume 34, Issue 11, November 2014, Pages 968-981
Journal: Nutrition Research - Volume 34, Issue 11, November 2014, Pages 968-981
نویسندگان
Rohit Sharma, Rajeev Kapila, Meena Kapasiya, Vamshi Saliganti, Gulshan Dass, Suman Kapila,