کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6075052 | 1203492 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Interferon-γ Protects from Staphylococcal Alpha Toxin-Induced Keratinocyte Death through Apolipoprotein L1
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
APOL1apolipoprotein L1RT-PCRa disintegrin and metalloproteinase 10ADAM10T helper type 2LC3Th2Real-time PCR - PCR به موقعSmall interfering RNA - RNA تداخل کوچکsiRNA - siRNAStat1 - sTAT1interferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینAtopic dermatitis - درماتیت آتوپیکlight chain 3 - زنجیره سبک 3lactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH signal transducer and activator of transcription 1 - مبدل سیگنال و فعال کننده رونویسی 1
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Staphylococcus aureus is a bacterial pathogen that frequently infects the skin, causing lesions and cell destruction through its primary virulence factor, alpha toxin. Here we show that interferon gamma (IFN-γ) protects human keratinocytes from cell death induced by staphylococcal alpha toxin. We find that IFN-γ prevents alpha toxin binding and reduces expression of the alpha toxin receptor, a disintegrin and metalloproteinase 10 (ADAM10). We determine that the mechanism for IFN-γ-mediated resistance to alpha toxin involves the induction of autophagy, a process of cellular adaptation to sublethal damage. We find that IFN-γ potently stimulates activation of the primary autophagy effector, light chain 3 (LC3). This process is dependent on upregulation of apolipoprotein L1. Depletion of apolipoprotein L1 by small interfering RNA significantly increases alpha toxin-induced lethality and inhibits activation of light chain 3. We conclude that IFN-γ plays a significant role in protecting human keratinocytes from the lethal effects of staphylococcal alpha toxin through apolipoprotein L1-induced autophagy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 136, Issue 3, March 2016, Pages 658-664
Journal: Journal of Investigative Dermatology - Volume 136, Issue 3, March 2016, Pages 658-664
نویسندگان
Anne M. Brauweiler, Elena Goleva, Donald Y.M. Leung,