کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6492033 | 43455 | 2012 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant protein production and streptomycetes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
SPaseMBAPSPSRPORFFBAPMFStreptomycesStrain improvement - بهبود فشارTAT - تاتFlux balance analysis - تجزیه و تحلیل تعادل شارMetabolic flux analysis - تجزیه و تحلیل شار متابولیکFermentation - تخمیر Protein secretion - ترشح پروتئینcoding sequence - توالی کدگذاریsignal recognition particle - ذرات شناخت سیگنالopen reading frame - قاب خواندن بازProton-motive force - نیروی حرکتی پروتونHeterologous protein - پروتئین Heterologousphage shock protein - پروتئین شوک فاژSignal peptide - پپتید سیگنالSignal peptidase - پپتیداز سیگنالCdS - کادمیم سولفید، سولفید کادمیم
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The biopharmaceutical market has come a long way since 1982, when the first biopharmaceutical product, recombinant human insulin, was launched. Just over 200 biopharma products have already gained approval. The global market for biopharmaceuticals which is currently valued at over US$99 billion has been growing at an impressive compound annual growth rate over the previous years. To produce these biopharmaceuticals and other industrially important heterologous proteins, different prokaryotic and eukaryotic expression systems are used. All expression systems have some advantages as well as some disadvantages that should be considered in selecting which one to use. Choosing the best one requires evaluating the options - from yield to glycosylation, to proper folding, to economics of scale-up. No host cell from which all the proteins can be universally expressed in large quantities has been found so far. Therefore, it is important to provide a variety of host-vector expression systems in order to increase the opportunities to screen for the most suitable expression conditions or host cell. In this overview, we focus on Streptomyces lividans, a Gram-positive bacterium with a proven excellence in secretion capacity, as host for heterologous protein production. We will discuss its advantages and disadvantages, and how with systems biology approaches strains can be developed to better producing cell factories.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biotechnology - Volume 158, Issue 4, 30 April 2012, Pages 159-167
Journal: Journal of Biotechnology - Volume 158, Issue 4, 30 April 2012, Pages 159-167
نویسندگان
Jozef Anné, Bárbara Maldonado, Jan Van Impe, Lieve Van Mellaert, Kristel Bernaerts,