کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7623962 | 1494550 | 2015 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Hurdles and pitfalls in measuring antioxidant efficacy: A critical evaluation of ABTS, DPPH, and ORAC assays
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کلمات کلیدی
ORACAAPHFRAPABTS•+CUPRACcupric ion reducing antioxidant capacityTEAC2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) - 2،2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)2,2′-azobis(2-amidinopropane) dihydrochloride - 2،2'-azobis (2-آمیدین پروپان) دی هیدروکلرایدHydrogen atom transfer - انتقال اتمی هیدروژنSET - تنظیمSingle electron transfer - تک الکترون انتقالTrolox equivalent antioxidant capacity - ظرفیت آنتی اکسیدانی معادل ترولوکسoxygen radical absorbance capacity - ظرفیت جذب رادیکال رادیکالLimitations - محدودیت هاinternational units - واحدهای بین المللیferric reducing antioxidant power - کاهش قدرت آنتی اکسیدان آهنHAT - کلاه
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Assays developed to measure radical scavenging ability of natural compounds have been used as a basis for ranking and recommending best foods for consumption. However, assays often were adapted for screening assays with inadequate consideration of reaction chemistry, particularly kinetics. Recent research results raise serious questions about the chemistry, execution, and application of these assays. This paper critically evaluates conceptual and technical issues that limit use and compromise validity of three commonly-used assays - TEAC/ABTS
- +, DPPH, and ORAC. Recommendations are made for discontinuing use of ABTS
- + and DPPH radicals for measuring radical quenching, redirecting them instead to distinguishing electron transfer reaction mechanisms. Conditions required for accurate results in ORAC are reviewed, and recommendations are made for redirecting this assay to distinguishing compounds that quench radicals by hydrogen atom transfer. The mechanistic information so gained can be then applied to understanding how natural antioxidants can be used most effectively in foods.
- +, DPPH, and ORAC. Recommendations are made for discontinuing use of ABTS
- + and DPPH radicals for measuring radical quenching, redirecting them instead to distinguishing electron transfer reaction mechanisms. Conditions required for accurate results in ORAC are reviewed, and recommendations are made for redirecting this assay to distinguishing compounds that quench radicals by hydrogen atom transfer. The mechanistic information so gained can be then applied to understanding how natural antioxidants can be used most effectively in foods.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Functional Foods - Volume 14, April 2015, Pages 111-125
Journal: Journal of Functional Foods - Volume 14, April 2015, Pages 111-125
نویسندگان
K.M. Schaich, X. Tian, J. Xie,