کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7625937 | 1494576 | 2018 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative analysis of the cyclic peptide GG-8-6 in rat plasma using LC-MS/MS and its application to a pharmacokinetic study
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
LLOQESILQCMQCRSDEtOAcHQCMRMACNHCC - HCCLC-MS/MS - LC-MS / MSEthyl acetate - اتیل استاتinternal standard - استاندارد داخلیAcetonitrile - استونیتریلUltraviolet - اشعه فرابنفشrelative standard deviation - انحراف استاندارد نسبیstandard deviation - انحراف معیارQuantitative analysis - تجزیه و تحلیل کمیlower limit of quantification - حد پایین کمیت سنجیMethanol - متانولPharmacokinetic study - مطالعه فارماکوکینتیکElectrospray ionization source - منبع یونیزاسیون ElectrosprayMeOH - مونmultiple reaction monitoring - نظارت چندگانه چندگانهCyclic peptide - پپتید سیکلیHepatocellular carcinoma - کارسینوم هپاتوسلولار(کارسینوم سلولهای استخوانی)high quality control - کنترل با کیفیت بالاquality control - کنترل کیفیتlow quality control - کنترل کیفیت پایین
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Hepatocellular carcinoma (HCC) is the second leading cause of cancer-related death worldwide. The discovery of new anticancer compounds is of great significance. GG-8-6, cyclo-(Val1-Leu2-Pro3-Ile4-Leu5-Leu6-Leu7-Val8-Leu9), a new synthetic cyclic peptide, might be a potential candidate for developing new anti-HCC drugs. GG-8-6 shares no structural homology to current anti-HCC drugs. Therefore, it was necessary to develop a quantitative method for the determination of GG-8-6 in vivo. Herein, a simple, specific and sensitive liquid chromatographic method with tandem mass spectrometric detection (LC-MS/MS) was developed and validated for the analysis of GG-8-6 in rat plasma. GG-8-6 and the internal standard (IS), A6, cyclo-(Val1-Leu2-Pro3-Ala4-Leu5-Leu6-Leu7-Val8-Leu9), were extracted from rat plasma by ethyl acetate. Chromatographic separation was performed on an Agilent Eclipse XDB-C18 column (4.6â¯Ãâ¯150â¯mm, 5â¯Î¼m) with a mobile phase consisting of acetonitrile-water containing 0.1% formic acid (90:10, v/v) with isocratic elution at a flow rate of 0.5â¯mL/min for 8.0â¯min. Multiple reaction monitoring (MRM) mode was performed with ion pairs of m/z: 974.8 â 861.8 for GG-8-6 and 932.7 â 819.8 for A6. The selectivity, matrix effects, recovery, intra- and inter-day precision and accuracy were validated with acceptable results in accordance with the US Food and Drug Administration guidelines. The calibration curve was linear (r2 > 0.99) over a concentration range of 1-1000â¯ng/mL with a lower limit of quantification (LLOQ) of 1â¯ng/mL. The method was successfully applied to the pharmacokinetic study of GG-8-6 in rats.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 159, 10 September 2018, Pages 217-223
Journal: Journal of Pharmaceutical and Biomedical Analysis - Volume 159, 10 September 2018, Pages 217-223
نویسندگان
Jie-Tao Chen, Xiao-Min Guo, Ping Yang, Qing Mu,