کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8634879 | 1569136 | 2018 | 31 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Bioinformatics profiling and expressional studies of microRNAs in root, stem and leaf of the bioenergy plant switchgrass (Panicum virgatum L.) under drought stress
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کلمات کلیدی
tRNAOryza sativaSbiprecursor miRNAsCGABNACCAqRT-PCRBrassica napusBrassica rapaZFNGMADCLLCRdsRNAFTPNIHsnRNAARFORFArabidopsis lyrataCRAmRNArRNApMRDNCBIRRMregulatory RNAsBLASTnblastxMFEUPEESTs - EST هاmessenger RNA - RNA messengerRibosomal RNA - RNA RibosomalRNA recognition motif - RNA تشخیص موتیفdouble-stranded RNA - RNA دو رشته ایsmall nucleolar RNA - RNA کوچک هسته ایAdenosine tri phosphate - آدنوزین تری فسفاتATP - آدنوزین تری فسفات یا ATPArabidopsis thaliana - آرابیدوپسیس تالیاناBasic Local Alignment Search Tool - ابزار جستجوی محلی سازگاری محلیAth - اتLet-7 - اجازه دهید 7Abiotic stress - استرس بی روحChlorogenic acid - اسید کلروژنیکALY - الیtransfer RNA - انتقال RNABlast - انفجارOsa - بخشExpressed sequence tags - تگ های توالی بیان شدهMinimum free energy - حداقل انرژی آزادRISC - خطرBiofuel crop - سوخت زیستیSorghum bicolor - سورگوم bicolorBra - سینه بندAuxin response factor - فاکتور پاسخ اکسینopen reading frame - قاب خواندن بازNational Institutes of Health - مؤسسات ملی بهداشتCAR - ماشینManihot esculenta - مانیوت اسکولنتاRNA Induced Silencing Complex - مجتمع خاموش کننده RNA منجر شده استNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیMeS - مسUTR یا untranslated regions - منطقه ترجمه نشدهMicroRNA - میکرو RNA MiRNA - میکروRNA، ریزآرانای، miRNAmap - نقشهNucleotide - نوکلئوتیدZinc finger protein - پروتئین انگشت رویmitogen-activated protein - پروتئین فعال mitogenfile transfer protocol - پروتکل انتقال فایلpre-miRNAs - پیش از miRNAsCoffea canephora - کافئا کانفوراCoffea arabica - کافیه عربیGlycine max - گلیسین حداکثر
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Switchgrass (Panicum virgatum L.) is a perennial fodder grass and well known as a model renewable bioenergy crop. The plant's high production of biomass for biofuel supply is due to its fast-growing and wide adaptation properties. Very little studies and data are available about microRNAs (miRNAs) in this important biofuel crop. This situation demands to focus and identify new miRNAs and also to study their expressional analysis. In current research, a combination of computational and expressional approaches is applied to profile and characterize miRNAs in switchgrass and analyze some of them under drought stress. A total of 158 new miRNAs belonging to 83 families are identified and characterized from switchgrass expressed sequence tags (ESTs) and genomic survey sequences (GSS). In addition, five pre-miRNA clusters as well as four sense and antisense pre-miRNAs are also predicted from these newly identified miRNAs. Furthermore, 39 miRNAs were randomly selected and subjected to quantitative real-time RT-PCR expressional studies in root, leaf and stem of switchgrass under drought stress. A set of 13, 20 and 25 miRNAs are found as drought responding miRNAs in root, stem and leaf respectively. A total of 894 putative targets are also predicted for 158 switchgrass' miRNAs. These targets play roles in metabolism, transcription factor, signaling pathways, growth & development, stress-related, transport protein and other vital processes. Such findings in switchgrass improved the baseline data of miRNAs and their targets. This data can be utilized to fine-tune this important bioenergy crop under biotic and abiotic stresses. Furthermore, it can also be used for the improvement of biofuel properties of the plant.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agri Gene - Volume 8, June 2018, Pages 1-8
Journal: Agri Gene - Volume 8, June 2018, Pages 1-8
نویسندگان
Muhammad Younas Khan Barozai, Zhujia Ye, Sasikiran Reddy Sangireddy, Suping Zhou,