کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8634907 | 1569136 | 2018 | 48 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In silico identification and characterization of microRNAs based on EST and GSS in orphan legume crop, Lens culinaris medik. (Lentil)
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کلمات کلیدی
LISPYRDAMFEPIP5KDHNGASAMFEIHmgGDAP1GSSCDKDehydrinbZIPABAMFENCBIERKRNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهBasic Local Alignment Search Tool - ابزار جستجوی محلی سازگاری محلیEST - استabscisic acid - اسید آبسزیکBlast - انفجارgenome survey sequence - توالی بررسی ژنومExpressed Sequence Tag - تکرار اظهار نظرFunctional annotation - حاشیه نویسی کاربردیbasic leucine zipper - زیپ پایه لوسینLentils - عدسphosphatidylinositol-4-phosphate 5-kinase - فسفاتیدیلینواستیل 4-فسفات 5-کینازNational Center for Biotechnology Information - مرکز ملی اطلاعات بیوتکنولوژیMicroRNA - میکرو RNA Gene ontology - هستیشناسی ژنیTarget gene - ژن هدفcyclin-dependent kinases - کیناز وابسته به سیکلینhigh mobility group - گروه تحرک بالا
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم دامی و جانورشناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
MicroRNAs (miRNAs) are a class of endogenous non-coding, small RNAs that are associated with the regulation of gene expression in eukaryotes. In plants, few miRNAs are highly conserved, that may have the same ancestor in the early stages of evolution. This fact allows the detection of conserved miRNAs in various plant species, especially in those that lack genome sequence information. Though the draft genome of the orphan crop, Lens culinaris Medik. (Lentil) is published, its complete genome assembly is still underway. In this computational study, an EST and GSS based comparative genomics approach were conducted to identify miRNAs in Lentils. The adopted approach was on the basis of a search for sequence similarity followed by series of filtering steps to provide reliable and precise results, eliminating the false-positive predictions. This study reports 24 miRNAs from 10,190 ESTs and 715 GSSs in Lentil. Further, it was also sought to pinpoint the 83 likely target genes of Lentil miRNAs and their most probable functions using Legume Information Resource (LIS) annotation pipeline. The newly identified miRNAs were mainly found to regulate the genes that encode transcription factors and key enzymes involved in metabolic processes as well as oxidation-reduction processes. Many of target genes were found to have an association with plant growth and development, stress response, defense and hormone signaling pathways. The miRNAs accounted is presumed to advance Lentil miRNAome in future as this computational study puts forward, first-ever, view towards miRNA research in Lentil.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Agri Gene - Volume 8, June 2018, Pages 45-56
Journal: Agri Gene - Volume 8, June 2018, Pages 45-56
نویسندگان
Vivek A.T.,