کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8715810 | 1587872 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
TRPA1 Acts in a Protective Manner in Imiquimod-Induced Psoriasiform Dermatitis in Mice
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کلمات کلیدی
TRPA1TRPV1TLRRTXIMQImiquimod - ایمی کیمودtumor necrosis factor-α - تومور نکروز عامل αToll-like receptor - تیالآرresiniferatoxin - رزینیفراتوکسینTNF-α - فاکتور نکروز توموری آلفاknockout - ناکاوتwild type - نوع وحشیtransient receptor potential ankyrin 1 - پتانسیل گیرنده گذرا ankyrin 1Transient receptor potential vanilloid 1 - پتانسیل گیرنده گذرا وانیلیوئید 1
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
امراض پوستی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
This study revealed the modulatory role of transient receptor potential ankyrin 1 (TRPA1) and vanilloid 1 (TRPV1) cation channels in the Aldara-induced (5% imiquimod) murine psoriasis model using selective antagonists and genetically altered animals. We have also developed a refined localized model to enable internal controls and reduce systemic effects. Skin pathology was quantified by measuring skin thickness, scaling, blood flow, and analyzing dermal cellular infiltrate, whereas nocifensive behaviors were also observed. Cytokine gene expression profiles were measured ex vivo. Psoriasiform dermatitis was significantly enhanced in TRPA1 knockout mice and with TRPA1 antagonist (A967079) treatment. By comparison, symptoms were decreased when TRPV1 function was inhibited. Imiquimod induced Ca2+ influx in TRPA1-, but not in TRPV1-expressing cell lines. Immunohistochemical studies revealed that CD4+ T helper cells express TRPA1 but not TRPV1 ion channels in mice skin. Compared with the TRPV1 knockout animals, additional elimination of the TRPA1 channels in the TRPV1/TRPA1 double knockout mice did not modify the outcome of the imiquimod-induced reaction, further supporting the dominant role of TRPV1 in the process. Our results suggest that the protective effects in psoriasiform dermatitis can be mediated by the activation of neuronal and nonneuronal TRPA1 receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Investigative Dermatology - Volume 138, Issue 8, August 2018, Pages 1774-1784
Journal: Journal of Investigative Dermatology - Volume 138, Issue 8, August 2018, Pages 1774-1784
نویسندگان
Ágnes Kemény, Xenia Kodji, Szabina Horváth, Rita Komlódi, Ãva SzÅke, Zoltán Sándor, Anikó Perkecz, Csaba Gyömörei, György Sétáló, Balázs Kelemen, Tamás BÃró, Balázs István Tóth, Susan D. Brain, Erika Pintér, Rolland Gyulai,