کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9028803 | 1130261 | 2005 | 19 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Role of cell signaling in B[a]P-induced apoptosis: characterization of unspecific effects of cell signaling inhibitors and apoptotic effects of B[a]P metabolites
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PI-3α-NFEGFPMSFAHRJnkTHFFBSCPPERKCyPPAHB[a]P - B [a] Pc-Jun N-terminal kinase - C-Jun N-terminal kinaseDMSO - DMSOMAPK - MAPKROS - ROSα-Naphthoflavone - α-نفتوفلوانBenzo[a]pyrene - بنزو [a] پییرنTetrahydrofuran - تتراهیدروفورانApoptosis - خزان یاختهایDimethyl sulfoxide - دیمتیل سولفواکسیدfoetal bovine serum - سرم جنین گاوCytochrome P450 - سیتوکروم پی۴۵۰epidermal growth factor - عامل رشد اپیدرمیphosphatidylinositol-3 - فسفاتیدیلینوزیتول 3phenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدMetabolism - متابولیسم Inhibitors - مهارکنندهPolycyclic aromatic hydrocarbons - هیدروکربن آروماتیک چندحلقهایmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استPropidium iodide - پروتئین یدیدMAP kinases - کیناز MAPextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیReactive oxygen species - گونههای فعال اکسیژنaryl hydrocarbon receptor - گیرنده آرویل هیدروکربن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Here we show that several cell signaling inhibitors have effect on cyp1a1 expression and the metabolism of benzo[a]pyrene (B[a]P) in Hepa1c1c7 cells. The CYP1A1 inhibitor α-naphthoflavone (α-NF), the p53 inhibitor pifithrin-α (PFT-α), the ERK inhibitors PD98059 and U0126, and the p38 MAPK inhibitors SB202190 and PD169316 induced the expression and level of cyp1a1 protein. On the other hand, during the first h the inhibitors appeared to reduce the metabolism of B[a]P as measured by the generation of tetrols and by covalent binding of B[a]P to macromolecules. In contrast, the phosphatidylinositol-3 (PI-3) kinase inhibitor wortmannin, had neither an effect on the cyp1a1 expression nor the B[a]P-metabolism. In order to avoid these unspecific effects, we characterized the mechanisms involved in the apoptotic effects of B[a]P-metabolites. B[a]P and the B[a]P-metabolites B[a]P-7,8-DHD and BPDE-I induced apoptosis, whereas B[a]P-4,5-DHD had no effect. B[a]P, B[a]P-7,8-DHD and BPDE-I induced an accumulation and phosphorylation of p53, while the Bcl-2 proteins Bcl-xl, Bad and Bid were down-regulated. Interestingly, the levels of anti-apoptotic phospho-Bad were up-regulated in response to B[a]P as well as to B[a]P-7,8-DHD and BPDE-I. Both p38 MAPK and JNK were activated, but the p38 MAPK inhibitors were not able to inhibit BPDE-I-induced apoptosis. PFT-α reduced the BPDE-I-induced apoptosis, while both the PI-3 kinase inhibitor and the ERK inhibitors increased the apoptosis in combination with BPDE-I. BPDE-I also triggered apoptosis in primary cultures of rat lung cells. In conclusion, often used cell signaling inhibitors both enhanced the expression and the level of cyp1a1 and more directly acted as inhibitors of cyp1a1 metabolism of B[a]P. However, studies with the B[a]P-metabolite BPDE-I supported the previous suggestion that p53 has a role in the pro-apoptotic signaling pathway induced by B[a]P. Furthermore, these studies also show that the reactive metabolites of B[a]P induce the anti-apoptotic signals, Akt and ERK. Neither the induction nor the activity of p38 MAPK and JNK seems to be of major importance for the B[a]P-induced apoptosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 151, Issue 2, 15 January 2005, Pages 101-119
Journal: Chemico-Biological Interactions - Volume 151, Issue 2, 15 January 2005, Pages 101-119
نویسندگان
Anita Solhaug, Steinar Ãvrebø, Steen Mollerup, Marit LÃ¥g, Per E. Schwarze, Stephen Nesnow, Jørn A. Holme,