کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9036574 | 1133346 | 2005 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Estrogenic and genotoxic potential of equol and two hydroxylated metabolites of Daidzein in cultured human Ishikawa cells
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کلمات کلیدی
S.DDAPIPMSFFCSDAIHprtEquolDTTHEPESFITCMuLVPBS-CMFDMEM17β-estradiol - 17β استرادیولBSA - BSADMSO - DMSODulbecco's modified Eagle's medium - Medal of Eagle اصلاح شده Dulbeccobovine serum albumin - آلبومین سرم گاوALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدEstrogen - استروژنstandard deviation - انحراف معیارIsoflavones - ایزوفلاونهاCrest - تاجTAE - توDaidzein - داایدزینdithiothreitol - دیتیوتریتولDimethyl sulfoxide - دیمتیل سولفواکسیدreverse transcription - رونویسی معکوسmicronuclei - ریزهستک یا میکرونوکلئوس fetal calf serum - سرم گوساله جنینIshikawa cells - سلول های Ishikawafluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتHydroxy - هیدروکسیpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازGEN - ژنرالEstrogen receptor - گیرنده استروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Estrogenic and genotoxic potential of equol and two hydroxylated metabolites of Daidzein in cultured human Ishikawa cells Estrogenic and genotoxic potential of equol and two hydroxylated metabolites of Daidzein in cultured human Ishikawa cells](/preview/png/9036574.png)
چکیده انگلیسی
The soy isoflavone daidzein (DAI) is known to undergo metabolism to equol (EQO) and to 3â²-hydroxy-DAI (3â²-HO-DAI) and 6-hydroxy-DAI (6-HO-DAI) in humans. In order to better understand the implications of soy diets for human health, the hormonal and genotoxic activities of these DAI metabolites were studied in cultured human endometrial carcinoma cells. When the estrogenicity was tested by cell-free binding to recombinant human estrogen receptor (ER) α and β as well as by the induction of enzyme activity and gene expression of alkaline phosphatase (ALP) in Ishikawa cells, the ranking order was EQO > DAI > 3â²-HO-DAI > 6-HO-DAI. All compounds had a higher affinity to ERβ than to ERα. No significant anti-estrogenic effects of the DAI metabolites were observed in the cells at non-cytotoxic concentrations. The in vitro genotoxicity was assessed by analyzing effects on cell cycle distribution and cell morphology as well as the induction of micronuclei (MN). EQO caused a slight increase in G1 and decrease in S phase of the cell cycle, and slightly but significantly induced kinetochore-positive as well as kinetochore-negative MN and an elevated proportion of abnormal mitotic spindles. 3â²-HO-DAI, but not 6-HO-DAI, induced kinetochore-negative MN. The observation that major human metabolites of DAI exhibit estrogenic and genotoxic potential may be of relevance for the safety evaluation of diets containing soy isoflavones.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology Letters - Volume 158, Issue 1, 28 July 2005, Pages 72-86
Journal: Toxicology Letters - Volume 158, Issue 1, 28 July 2005, Pages 72-86
نویسندگان
L. Lehmann, H.L. Esch, J. Wagner, L. Rohnstock, M. Metzler,