کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9485723 | 1329286 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of silicon on H+-ATPase and H+-PPase activity, fatty acid composition and fluidity of tonoplast vesicles from roots of salt-stressed barley (Hordeum vulgare L.)
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کلمات کلیدی
PVP8-anilino-1-naphthalene-sulfonic acidHEPESDTTN-2-hydroxyethyl piperazine-N′-2-ethanesulfonic acidEGTATCAH+-ATPase activityTRISPMSFBSA - BSAbovine serum albumin - آلبومین سرم گاوtrichloroacetic acid - اسید ترشکلراکتیکSalt stress - تنش شوریBarley (Hordeum vulgare L.) - جو (Hordeum vulgare L.)dithiothreitol - دیتیوتریتولANS - سالsilicon - سیلیسیم polyvinylpyrrolidone - پلی وینیل پیرولیدون
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We investigated the effects of silicon (Si) on time-dependent changes in root tonoplast H+-ATPase and H+-PPase activities, membrane fatty acid compositions and tonoplast fluidity in two barley (Hordeum vulgare L.) cultivars differing in salt tolerance. Plants were grown in NaCl-free (control) and NaCl-supplied (60 and 120Â mM, respectively) nutrient solutions with or without 1.0Â mM Si. Plant roots were harvested to isolate tonoplast vesicles for assay of H+-ATPase and H+-PPase activities at days 2, 4, and 6 after treatment in the first experiment and for analysis of membrane fatty acid composition and fluidity at day 4 after treatment in the second experiment. The results showed that tonoplast H+-ATPase and H+-PPase activities in roots of salt-treated plants increased at day 2, which was more obvious at 60Â mM NaCl in the salt-tolerant cultivar than in the salt-sensitive cultivar, and then decreased at day 4 and onward. These enzyme activities decreased consistently from days 2 to 6 for treatment with 120Â mM NaCl. However, inclusion of 1.0Â mM Si significantly enhanced both H+-ATPase and H+-PPase activities in roots of salt stressed barley, which was irrespective of NaCl level or cultivar used. The ratio of unsaturated to saturated fatty acids (U/S) increased under salt stress for both cultivars. Addition of Si to salt treatment increased the ratio of U/S in salt-tolerant cultivar but it did not in salt-sensitive cultivar compared to non-Si-amended salt treatment. Salt treatment decreased tonoplast fluidity of roots of barley significantly compared with control treatment. However, root tonoplast fluidity was significantly lower in the Si-amended salt treatment than in the non-Si-amended salt treatment. These results were in line with the previous findings that Si could help increase antioxidative defense and reduce membrane lipid oxidative damage in barley under salt stress. The possible mechanisms involved in Si-enhanced salt tolerance were discussed with respect to cell membrane integrity, stability and function in barley.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental and Experimental Botany - Volume 53, Issue 1, February 2005, Pages 29-37
Journal: Environmental and Experimental Botany - Volume 53, Issue 1, February 2005, Pages 29-37
نویسندگان
Yongchao Liang, Wenhua Zhang, Qin Chen, Ruixing Ding,