کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9908678 | 1547708 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
E3B1, a human homologue of the mouse gene product Abi-1, sensitizes activation of Rap1 in response to epidermal growth factor
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کلمات کلیدی
GEFsPDGFRSH2PP1PDGFRAP1PABC3GSH3GSTRACmAbABI-1EGFSTI 571EGFRSrc tyrosine kinase - Src تیروزین کینازMonoclonal antibody - آنتی بادی مونوکلونالPolyclonal antibody - آنتی بادی های پلی کلونالGAP - شکافepidermal growth factor - عامل رشد اپیدرمیGuanine nucleotide exchange factors - عوامل مبادله نوکلئوتیدی گوانینplatelet-derived growth factor - فاکتور رشد حاصل از پلاکتSrc Homology 2 - همبستگی Src 2Src homology 3 - همبستگی Src 3GTPase-activating protein - پروتئین فعال GTPaseglutathione-S-transferase - گلوتاتیون S-ترانسفرازEGF receptor - گیرنده EGFplatelet-derived growth factor receptor - گیرنده عامل فاکتور رشد یافته پلاکتEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
E3B1, a human homologue of the mouse gene product Abi-1, has been implicated in growth-factor-mediated regulation of the small GTPases p21Ras and Rac. E3b1 is a regulator of Rac because it can form a complex with Sos-1 and eps8, and such a Sos-1-e3B1-eps8 complex serves as a guanine nucleotide exchange factor for Rac. In the present study, we found that overexpression of e3B1 in NIH3T3/EGFR cells sensitized EGF-induced activation of Rac1, whereas it had no impact on EGF-induced activation of p21Ras. Remarkably, we found that EGF-induced activation of the p21Ras-related GTPase Rap1 was also sensitized in NIH3T3/EGFR-e3B1 cells. Thus, in NIH3T3/EGFR-e3B1 cells, maximal EGF-induced activation of Rap1 occurs with a dose of EGF much lower than in NIH3T3/EGFR cells. We also report that overexpression of e3B1 in NIH3T3/EGFR cells renders EGF-induced activation of Rap1 completely dependent on Src tyrosine kinases but not on c-Abl. However, EGF-induced tyrosine phosphorylation of the Rap GEF C3G occurred regardless of whether e3B1 was overexpressed or not, and this did not involve Src tyrosine kinases. Accordingly, we propose that overexpression of e3B1 in NIH3T3/EGFR cells leads to mobilization of Src tyrosine kinases that participate in EGF-induced activation of Rap1 and inhibition of cell proliferation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 310, Issue 2, 1 November 2005, Pages 463-473
Journal: Experimental Cell Research - Volume 310, Issue 2, 1 November 2005, Pages 463-473
نویسندگان
Veronika Jenei, Tommy Andersson, Judit Jakus, Karim Dib,