کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10158352 | 1666522 | 2018 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Protective effect of wax apple (Syzygium samarangense (Blume) Merr. & L.M. Perry) against streptozotocin-induced pancreatic Ã-cell damage in diabetic rats
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کلمات کلیدی
b.w.CATSTZp.o.FBGIL-1IL-6DABHRPHOMA-BBcl-2i.p.NF-κBRNS3,3′-diaminobenzidine - 3،3'-diaminobenzidineBSA - BSAGSH-Px - GSH-PXH&E - H & Emitochondrial outer membrane permeabilization - permeabilization غشای خارجی بیرونی میتوکندریROS - ROSbovine serum albumin - آلبومین سرم گاوOral administration - اداره دهان و دندانstreptozotocin - استرپتوزوتوسینinterleukin-6 - اینترلوکین ۶interleukin-1 - اینترلوکین-1Diabetes - بیماری قندintraperitoneal injection - تزریق داخل صفاقیEnzyme-linked immunosorbent assay - تست الیزاELISA - تست الیزاOxidative stress - تنش اکسیداتیوtumor necrosis factor-alpha - تومور نکروز عامل آلفاApoptosis - خزان یاختهایDiabetes mellitus - دیابت قندیSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازTNF-α - فاکتور نکروز توموری آلفاnuclear factor kappa-B - فاکتور هسته ای کاپا-Bfasting blood glucose - قند خون ناشتاB-cell lymphoma 2 - لنفوم سلول B 2MOMP - ماموریتHematoxylin and Eosin - هماتوکسیلین و ائوزینbody weight - وزن بدنHorseradish peroxidase - پراکسیداز هوررادیشoptical density - چگالی نوریCatalase - کاتالازreactive nitrogen species - گونه های واکنش پذیر نیتروژنReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم پزشکی و سلامت
پزشکی و دندانپزشکی
تومور شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The purpose of this study was to investigate the protective properties and mechanisms of wax apple (Syzygium samarangense (Blume)) against streptozotocin (STZ)-induced pancreatic Ã-cell apoptosis in diabetic rats. Diabetes was induced by STZ (65âmg/kg; i.p.) injection and wax apple (100âmg/kg) was orally administered to diabetic rats for a period of 30 days. During this time, fasting blood glucose (FBG) and body weight were measured weekly. At the end of the experiment, serum insulin, HOMA-B, and pancreatic insulin expression were assessed. The expression of apoptosis-related proteins along with the nitrotyrosine level, antioxidant activities, and pro-inflammatory cytokine TNF-α in the pancreas were also determined. STZ-induced diabetic rats exhibited an increase in FBG, and a decrease in body weight, serum and pancreatic insulin, as well as HOMA-B. Pancreatic apoptosis was noted in diabetic rats and indicated by enhancing the expression of cleaved caspase-3 and Bax proteins and downregulating the expression of Bcl-2 and Bcl-xl proteins. The activities of antioxidant CAT and SOD in the pancreas of the diabetic rats was also reduced. Importantly, wax apple treatment resulted in a significant reduction of FBG and increased body weight in diabetic rats. Wax apple also improved pancreatic Ã-cell function, this was clearly evidenced by increased HOMA-B and pancreatic and serum insulin levels in diabetic rats. Moreover, pancreatic Ã-cell apoptosis was alleviated with significantly down-regulated cleaved caspase-3 and Bax protein expression, and upregulated Bcl-2 and Bcl-xl protein expression in wax apple treated diabetic rats. These were related to the induction of CAT and SOD activities, and reduction of nitrotyrosine and TNF-α levels in wax apple administration. Overall, these results provide evidence that wax apple protects against STZ-induced pancreatic β-apoptosis and dysfunction in diabetic rats, possibly through inhibiting oxidative stress and pro-inflammatory cytokine, and activating anti-apoptotic proteins.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomedicine & Pharmacotherapy - Volume 108, December 2018, Pages 634-645
Journal: Biomedicine & Pharmacotherapy - Volume 108, December 2018, Pages 634-645
نویسندگان
Aree Khamchan, Tatdao Paseephol, Wanthanee Hanchang,