کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10814998 | 1058441 | 2016 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gene expression profiling reveals the role of RIG1 like receptor signaling in p53 dependent apoptosis induced by PUVA in keratinocytes
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
TBKmelanoma differentiation associated gene 5IKKεMAVSMDA5IRF3RIG-1TP533'UTRmTORFASp533′ untranslated region - 3 منطقه غیر ترجمهMdm2 - MDM2v-akt murine thymoma viral oncogene homolog 1 - v-ACT ویروس آنفولانزای ویروسی ویروسی توموما 1Akt - آکتtumor protein p53 - تومور پروتئین p53TANK-binding kinase 1 - تین کیناز 1Apoptosis - خزان یاختهایmurine double minute 2 - دوقلوهای قهوه ای 2Interferon regulatory factor 3 - عامل تنظیمی اینترفرون 3Mechanistic target of rapamycin - هدف مکانیکی رپامایسینmitochondrial antiviral signaling protein - پروتئین سیگنالینگ ضد ویروسی میتوکندریPsoriasis - پسوریازیسPuva - پیوا
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Photochemotherapy using 8-methoxypsoralen in combination with UVA radiation (PUVA) is an effective treatment for various skin dermatosis including psoriasis however its molecular mechanism is not clear. Previously we demonstrated that PUVA differentially regulates miRNA expression profile with a significant up-regulation of hsa-miR-4516. To study in detail the molecular mechanism of PUVA in keratinocytes, we investigated the genome wide transcriptomic changes using Illumina whole genome gene expression beadchip. Microarray analysis revealed 1932 differentially expressed gene and their Insilico analysis revealed Retinoic Acid Inducible Gene-I (RIG-1) signaling, apoptosis and p53 pathway to be associated with PUVA induced effects. We demonstrate that miR-4516 mediated down-regulation of UBE2N promotes p53 nuclear translocation and pro-apoptotic activity of PUVA is independent of IRF3 but is mediated by the RIG-I in a p53 and NFκB dependent manner. Additionally, PUVA inactivated the AKT/mTOR pathway in concert with inhibition of autophagy and suppressed cell migration. Taken together this study broadens our understanding about the mechanism of action of PUVA providing possible new strategy targeting proapoptotic function of RIG-1, a regulator of innate immune response or p53 for psoriasis therapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cellular Signalling - Volume 28, Issue 1, January 2016, Pages 25-33
Journal: Cellular Signalling - Volume 28, Issue 1, January 2016, Pages 25-33
نویسندگان
Shruti Chowdhari, Neeru Saini,