کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10904239 | 1086568 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
eIF4E-Overexpression imparts perillyl alcohol and rapamycin-mediated regulation of telomerase reverse transcriptase
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کلمات کلیدی
PI3KUTReIF4PVDFEtOH4E-binding protein 1mTORp70 S6 kinaseS6KPOHPI3K-Akt-mTOR pathway4E-BP1RAPA3-hydroxy-3-methylglutaryl-CoA reductase - 3-هیدروکسی-3-methylglutaryl-CoA ردوکتازHMG-CoA reductase - HMG-CoA ردوکتازEthanol - اتانولOncogene addiction - اعتیاد Oncogeneperillyl alcohol - الکل پریللیCho - برایTERT - تترChinese Hamster Ovary - تخمدان هامستر چینیTelomerase reverse transcriptase - ترکیبریت معکوس تلومرازP-bodies - جسد PRapamycin - راپامایسینphosphoinositide 3-kinase - فسفینوزیتید 3-کینازTelomerase activity - فعالیت تلومرازuntranslated region - منطقه غیر ترجمهMechanistic target of rapamycin - هدف مکانیکی رپامایسینPolyvinylidene fluoride - پلی وینیلیدین فلورایدCHAPS - چاپس
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: eIF4E-Overexpression imparts perillyl alcohol and rapamycin-mediated regulation of telomerase reverse transcriptase eIF4E-Overexpression imparts perillyl alcohol and rapamycin-mediated regulation of telomerase reverse transcriptase](/preview/png/10904239.png)
چکیده انگلیسی
Translation is mediated partly by regulation of free eukaryotic initiation factor 4E (eIF4E) levels through PI3K-Akt-mTOR signaling. Cancer cells treated with the plant-derived perillyl alcohol (POH) or the mechanistic target of rapamycin (mTOR) inhibitor rapamycin dephosphorylate eIF4E-binding protein (4E-BP1) and attenuate cap-dependent translation. We previously showed in cancer cell lines with elevated eIF4E that POH and rapamycin regulate telomerase activity through this pathway. Here, immortalized Chinese hamster ovary (CHO) control cells and CHO cells with forced eIF4E expression (rb4E) were used to elucidate eIF4E's role in telomerase regulation by POH and rapamycin. Despite 5-fold higher eIF4E amounts in rb4E, telomerase activity, telomerase reverse transcriptase (TERT) mRNA, and TERT protein were nearly equivalent in control and rb4E cells. In control cells, telomerase activity, TERT mRNA and protein levels were unaffected by either compound. In contrast, telomerase activity and TERT protein were both attenuated by either agent in rb4E cells, but without corresponding TERT mRNA decreases indicating a translational/post-translational process. S6K, Akt, and 4E-BP1 were modulated by mTOR mediators only in the presence of increased eIF4E. Thus, eIF4E-overexpression in rb4E cells enables inhibitory effects of POH and rapamycin on telomerase and TERT protein. Importantly, eIF4E-overexpression modifies cellular protein synthetic processes and gene regulation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Cell Research - Volume 319, Issue 13, 1 August 2013, Pages 2103-2112
Journal: Experimental Cell Research - Volume 319, Issue 13, 1 August 2013, Pages 2103-2112
نویسندگان
Tabetha Sundin, Dennis Peffley, Patricia Hentosh,