کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1983647 | 1539926 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Reactive oxygen species participate in the p38-mediated apoptosis induced by potassium deprivation and staurosporine in cerebellar granule neurons
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کلمات کلیدی
MAPKKKCGNp38ATF2KMPSB203580MAPKKCATPotassium deprivationFDAFeTPPSK25DHET - dhetMAPKs - MAPK هاMAPK kinase - MAPK کینازMAPK kinase kinase - MAPK کیناز کینازNOx - NOXAntioxidants - آنتی اکسیدانstaurosporine - استوسوسورپینNADPH oxidase - اکسیداز NADPH Apoptosis - خزان یاختهایdihydroethidium - دی هیدروتیدیمDIV - دیوdays in vitro - روز in vitroSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازactivating transcription factor-2 - فعال کردن عامل رونویسی 2cerebellar granule neurons - نورونهای گرانشی مخچهHigh potassium - پتاسیم بالاPropidium iodide - پروتئین یدیدKaempferol - کائمفرولCatalase - کاتالازcaspase - کسپاز یا کاسپازmitogen-activated protein kinases - کیناز پروتئین فعال MitogenReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Apoptosis induced by low potassium (K5) or staurosporine in cerebellar granule neurons triggers an increase in reactive oxygen species (ROS) levels. ROS inhibition by antioxidants or inhibitors of the NADPH oxidase (NOX) activity reduces the apoptosis induced by both stimuli. It has been reported that JNK mediates the apoptosis induced by K5 but not by staurosporine. No information is available about the role of other signaling pathways such as p38 in staurosporine-induced apoptosis, and whether p38 activation could be related to ROS levels induced by both K5 and staurosporine. Here, we explored this possibility and found that K5 activates p38 and ATF2 and that the inhibition of p38 activity prevents the apoptosis induced by this treatment. We also found that p38 is downstream of ROS generation induced by K5. On the other hand, staurosporine promotes a sustained activation of p38. We found that p38 inhibition markedly decreases ROS generation, NOX activity and apoptosis induced by staurosporine. Furthermore, antioxidants inhibit p38 activation induced by staurosporine. These data indicate that apoptosis induced by both K5 and staurosporine is dependent on p38 activation, which is mediated by ROS. In addition, p38 activation by staurosporine induces a further production of ROS through NOX activation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: The International Journal of Biochemistry & Cell Biology - Volume 43, Issue 9, September 2011, Pages 1373-1382
Journal: The International Journal of Biochemistry & Cell Biology - Volume 43, Issue 9, September 2011, Pages 1373-1382
نویسندگان
YazmÃn Ramiro-Cortés, Alicia Guemez-Gamboa, Julio Morán,