کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5844796 | 1561049 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intracerebroventricular administration of ouabain, a Na/K-ATPase inhibitor, activates mTOR signal pathways and protein translation in the rat frontal cortex
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
eIF4BNMDAN-methyl-d-aspartateintracerebroventricularICVp70S6KPLCPKCaCSFDAPIERKmTORPMS4E-BPNa-pumpEGFR4′,6′-diamidino-2-phenylindole - 4 '، 6'-diamidino-2-phenylindoleBDNF - BDNF یا فاکتور نورونزایی مشتقشده از مغز MAPK - MAPKNa/K-ATPase - Na / K-ATPasep70 ribosomal S6 kinase - P6O سینوسی سدیم ریبوزومیbipolar disorder - اختلال دو قطبیanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceProtein synthesis - سنتز پروتئینextracellular signal regulated kinase - سیگنال خارج سلولی kinase را تنظیم می کندBrain-derived neurotrophic factor - فاکتور نوروتروفی مشتق شده از مغزphospholipase C - فسفولیپاز Cartificial cerebrospinal fluid - مایع مغزی نخاعی مصنوعیProtein kinase C - پروتئین کیناز سیmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenoptical density - چگالی نوریEpidermal growth factor receptor - گیرنده فاکتور رشد اپیدرمال
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
روانپزشکی بیولوژیکی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Intracerebroventricular administration of ouabain, a Na/K-ATPase inhibitor, activates mTOR signal pathways and protein translation in the rat frontal cortex Intracerebroventricular administration of ouabain, a Na/K-ATPase inhibitor, activates mTOR signal pathways and protein translation in the rat frontal cortex](/preview/png/5844796.png)
چکیده انگلیسی
Intracerebroventricular (ICV) injection of ouabain, a specific Na/K-ATPase inhibitor, induces behavioral changes in rats in a putative animal model of mania. The binding of ouabain to Na/K-ATPase affects signaling molecules in vitro, including ERK1/2 and Akt, which promote protein translation. We have also reported that ERK1/2 and Akt in the brain are involved in the ouabain-induced hyperactivity of rats. In this study, rats were given an ICV injection of ouabain, and then their frontal cortices were examined to determine the effects of ouabain on the mTOR/p70S6K/S6 signaling pathway and protein translation, which are important in modifications of neural circuits and behavior. Rats showed ouabain-induced hyperactivity up to 8Â h following injection, and increased phosphorylation levels of mTOR, p70S6K, S6, eIF4B, and 4E-BP at 1, 2, 4, and 8Â h following ouabain injection. Immunohistochemical analyses revealed that increased p-S6 immunoreactivity in the cytoplasm of neurons by ouabain was evident in the prefrontal, cingulate, and orbital cortex. These findings suggested increased translation initiation in response to ouabain. The rate of protein synthesis was measured as the amount of [3H]-leucine incorporation in the cell-free extracts of frontal cortical tissues, and showed a significant increase at 8Â h after ouabain injection. These results suggest that ICV injection of ouabain induced activation of the protein translation initiation pathway regulated by ERK1/2 and Akt, and prolonged hyperactivity in rats. In conclusion, protein translation pathway could play an important role in ouabain-induced hyperactivity in a rodent model of mania.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Progress in Neuro-Psychopharmacology and Biological Psychiatry - Volume 45, 1 August 2013, Pages 73-82
Journal: Progress in Neuro-Psychopharmacology and Biological Psychiatry - Volume 45, 1 August 2013, Pages 73-82
نویسندگان
Se Hyun Kim, Hyun-Sook Yu, Hong Geun Park, Kyooseob Ha, Yong Sik Kim, Soon Young Shin, Yong Min Ahn,