کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5847922 | 1561612 | 2015 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Gastroprotective effect of crocin in ethanol-induced gastric injury in rats
ترجمه فارسی عنوان
اثر گاستروپرتوتیک کروکین در آسیب ناشی از اتانول معده در موش صحرایی
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کلمات کلیدی
hsp70GSHGAPDHPNAPGE2DPPHqPCRIL-68-OH-dGγ-GCSNF-κBMDANSAIDS2,2-diphenyl-1-picrylhydrazyl - 2،2-دی فینیل-1-پریکیل هیدرازیل8-hydroxy-2′-deoxyguanosine - 8-هیدروکسی-2'-دگزسی گوانوزینCrocin - CrocianMPO - DFOH&E - H & Ep-Nitroanilide - p-nitroanilideROS - ROSinflammation - التهاب( توروم) interleukin-6 - اینترلوکین ۶Oxidative stress - تنش اکسیداتیوtumor necrosis factor-alpha - تومور نکروز عامل آلفاApoptosis - خزان یاختهایNon-steroidal anti-inflammatory drugs - داروهای ضد التهابی غیر استروئیدیGUI - رابط کاربری گرافیکیSOD - سدPC-12 cells - سلول های PC-12Superoxide dismutase - سوکسوکس دیسموتازTNF-α - فاکتور نکروز توموری آلفاnuclear factor kappa B - فاکتور هسته ای کاپا Bmalondialdehyde - مالون دی آلدهیدmyeloperoxidase - میلوپراکسیداز Hematoxylin and Eosin - هماتوکسیلین و ائوزینquantitative polymerase chain reaction - واکنش زنجیره ای پلیمراز کمیheat shock protein 70 - پروتئین شوک حرارت 70Prostaglandin E2 - پروستاگلاندین E2reduced glutathione - کاهش گلوتاتیونglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
چکیده انگلیسی
The present study investigated the gastroprotective effect of crocin in ethanol-induced gastric injury in rats. Rats were allocated into a normal group, an ulcer group, a crocin-treated group, an ulcer group pretreated with crocin, and an ulcer group pretreated with omeprazole as a reference anti-ulcer drug. Rats were sacrificed 3 h after ethanol administration. Prophylactic administration of crocin (50 mg/kg/day, i.p.) for 3 consecutive days before the administration of 70% ethanol (10 ml/kg, orally) resulted in significant gastroprotection compared to ethanol-ulcerated rats as manifested by significant reduction in the gastric ulcer index. Crocin pretreatment increased ethanol-lowered levels of gastric juice mucin and mucosal prostaglandin E2 (PGE2) and interleukin-6 (IL-6). Moreover, crocin significantly decreased ethanol-elevated tumor necrosis factor-alpha (TNF-α) level, myeloperoxidase activity and heat shock protein 70 mRNA and protein levels. It also restored ethanol-altered mucosal levels of glutathione, malondialdehyde and superoxide dismutase activity. Furthermore, crocin-pretreatment alleviated ethanol-induced mucosal apoptosis as revealed by significant down-regulation of cytochrome c and caspase-3 mRNA expression, significant decrease in caspase-3 activity and mitigated DNA fragmentation as indicated by significant decrements in comet parameters. The protective efficacy of crocin was further supported by histological assessment. No significant difference was observed between crocin and omeprazole (20 mg/kg orally 1 h before ethanol administration) regarding their mucin-secretagogue and antioxidant effects, as well as their effects on TNF-α, IL-6 and cytochrome c. On the other hand, omeprazole was superior in enhancing PGE2 level and in alleviating neutrophil infiltration, caspase-3 activation and DNA fragmentation. Conclusively, crocin protects rat gastric mucosa against ethanol-induced injury via anti-inflammatory, anti-oxidative, anti-apoptotic and mucin-secretagogue mechanisms that are probably mediated by enhanced PGE2 release.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 229, 5 March 2015, Pages 26-35
Journal: Chemico-Biological Interactions - Volume 229, 5 March 2015, Pages 26-35
نویسندگان
Shohda A. El-Maraghy, Sherine M. Rizk, Nancy N. Shahin,