کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5901050 | 1568902 | 2014 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Transcriptomics profiling and steroid production in mummichog (Fundulus heteroclitus) testes after treatment with 5α-dihydrotestosterone
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کلمات کلیدی
DHTGSICelEDCCatechol-O-methyltransferaseLSIFASmTORRhoAAnxa1GPTFathead minnowESDCOMTCD40MSNFAAHFADDCcl11GBANBFBNIP3PSAPNAMPTANGPTL3angiopoietin-like 3Fis1CeruloplasminMPST11β-hydroxysteroid dehydrogenaseGRIA2apoBhLFAMACRCYP1B1Irs2ANGPTL4ABCC2ADARPNPLA3CBR1DHCR24GPx1CXCL10CtsBNFE2L2MTTPGNMTHNF4AEPXCCL25CD81GSTT1PPLLCATABCG8EIF2AK4PLTPapoMMafBEsterase Dglutathione S-transferase theta 1APOA4FLNADGAT1CYP11A1d-dopachrome tautomeraseEPHX1CTSKGRB2NPBAKR1A1ATP7ARPSAITIH4ProsaposinFHMACY1Aminoacylase 1GPx4NR1D1AGXTHSPG2BLNKGSTZ1Mercaptopyruvate sulfurtransferaseCYP3A7CYP8B1RASD1RGS5HpxABCC3APOC1ATR interacting proteinRAB18CD53MoesinKLF1ALDH3A1SPI1Selenoprotein TEphB2Alpha-methylacyl-CoA racemasemethionine sulfoxide reductase B1Apolipoprotein C-IFli1Neuropeptide BFABP2GPR1PARK7MCAMeEF2KALDH3A2NR3C2Dnm1GSTM3PZPdiacylglycerol o-acyltransferase 1PRDX3PRDX5BCL2/adenovirus E1B 19 kDa interacting protein 3GPx7Prdx1PTGS111KTDCXRCYP4B1ras homolog family member AB-Cell LinkerSGPL1eukaryotic elongation factor-2 kinase11-ketotestosterone - 11-کتواستاسترون24-dehydrocholesterol reductase - 24-دی هیدروکلوسترول ردوکتاز5α-Dihydrotestosterone - 5α-دی هیدروتستوسترونHIF1a - HIF1Mcl1 - MCL1alanine-glyoxylate aminotransferase - آلانین گلیوکسیلات آمینوترانسفرازangiopoietin-like 4 - آنژیوپوئیتین مانند 4Annexin A1 - آنیلیون A1Apolipoprotein A-IV - آپولیپوپروتئین A-IVApolipoprotein B - آپولیپوپروتئین BApolipoprotein M - آپولیپوپروتئین مeosinophil peroxidase - ائوزینوفیل پراکسیدازAHNAK - احنکFatty acid amide hydrolase - اسید آمینه هیدرولاز اسید چربdissolved oxygen - اکسیژن محلولEIA - اینInsulin receptor substrate 2 - بستر گیرنده انسولین 2Pir - بیش از حدSub-network enrichment analysis - تجزیه و تحلیل غنی سازی زیر شبکهendocrine disrupting compound - ترکیبات غشایی مصنوعیcomplement component 5 - جزء مکمل 5CFD - دینامیک سیالاتDynamin 1 - دینین 1DDT - دیکرو دیفنیل تری کلرواتانRORA - راراATRIP - سفرLiver somatic index - شاخص سمی کبدhepatic leukemia factor - عامل لوسمی کبدیnuclear factor (erythroid-derived 2)-like 2 - فاکتور هسته ای (erythroid-derived 2) -like 2Condition factor - فاکتور وضعیتneutral buffered formalin - فرمالین بافر خنثیlecithin-cholesterol acyltransferase - لسیتین-کلسترول آسیل ترانسفرازLipc - لیپسmelanoma cell adhesion molecule - مولکول چسبندگی سلولی ملانومSelS - نمکnicotinamide phosphoribosyltransferase - نیکوتین آمید فسفریبوسیل ترانسفرازMechanistic target of rapamycin - هدف مکانیکی رپامایسینHemopexin - هموکسینpatatin-like phospholipase domain containing 3 - پاتین مانند فسفولیپاز حاوی 3peroxiredoxin 3 - پراکسی اوردوکسین 3Peroxiredoxin 5 - پراکسی اوردوکسین 5Peroxiredoxin 1 - پراکسیردوکسین 1microsomal triglyceride transfer protein - پروتئین انتقال تری گلیسرید میکروسمالیPhospholipid transfer protein - پروتئین انتقال فسفولیپیدgrowth factor receptor-bound protein 2 - پروتئین گیرنده پروتئینی 2 عامل رشدperiplakin - پری پلاتینCathepsin B - کاتئفسین BCathepsin K - کتهفسین کcarbonyl reductase 1 - کربونیل ردوکتاز 1choline kinase - کولین کینازglutathione peroxidase 1 - گلوتاتیون پراکسیداز 1glutathione peroxidase 4 - گلوتاتیون پراکسیداز 4glycine N-methyltransferase - گلیسین N-متیل ترانسفرازleukotriene B4 receptor - گیرنده B4 لوکوترین
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
علوم غدد
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
5α-Dihydrotestosterone (DHT) is a potent androgen in mammals with multiple roles; however the physiological actions of DHT in male fishes are not well known. To address this knowledge gap, male mummichog (Fundulus heteroclitus) were continuously exposed to 0, 5, and 50 μg/L DHT for 21 days. Following exposure, testes were separated for histology, ex vivo incubation to measure steroidogenic capacity, and gene expression analyses (real-time PCR and microarray). DHT significantly decreased ex vivo 11-ketotestosterone (11KT) production in males exposed to 50 μg/L DHT but not 5 μg/L DHT, and DHT exposure did not affect ex vivo testosterone production. Histological examination revealed that the amount of interlobular and connective tissue present in the testes was increased in the 50 μg/L DHT treatment. Despite reductions in the production of 11KT, DHT did not affect the expression of targeted genes in the steroidogenic pathway such as steroidogenic acute regulatory protein (star), P450 side chain cleavage (cyp11a1) and 11β-hydroxysteroid dehydrogenase (hsd11b3). Microarray analysis in the testes of individuals from control and 50 μg/L DHT revealed that males exposed to 50 μg/L DHT showed regulated transcriptional sub-networks that were related to immunity, regulation of blood flow, lipids and xenobiotic clearance, suggesting that DHT may be involved in the physiological regulation of these processes in the fish testes. A second objective of this study was to determine the feasibility of measuring mRNA levels in tissues used for ex vivo steroid production by comparing RNA integrity and transcript levels in testes of both immediately flash frozen tissue and incubated tissue. There was no significant difference in RNA quality between the two time points, indicating RNA integrity can remain intact for at least 18 h in ex vivo assays, thereby providing a viable option for researchers assessing multi-level biological reproductive endpoints when limited tissue is available. While the gene expression levels of actb, efla, rps12, rps18, star, and hsd11b3 remained unchanged, esr2a (esrba), esr2b (esrbb) and cyp11a1 were significantly lower in incubated tissue compared to flash frozen tissue. Therefore caution must be used as the steady-state levels of select genes may change over time. This study improves our understanding of DHT action in the teleostean testis and generates new hypotheses regarding cell processes that are regulated by this underexplored and potent androgen.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: General and Comparative Endocrinology - Volume 203, 1 July 2014, Pages 106-119
Journal: General and Comparative Endocrinology - Volume 203, 1 July 2014, Pages 106-119
نویسندگان
A. Feswick, J.S. Ings, M.A. Doyle, T. Bosker, K.R. Munkittrick, C.J. Martyniuk,