کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8498231 | 1553594 | 2018 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
The role of Nrf2-Keap1 signaling pathway in the antioxidant defense response induced by PAHs in the calm Ruditapes philippinarum
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کلمات کلیدی
PBSMDAR. philippinarumKelch-like-ECH-associated protein 17-ethoxyresorufin-O-deethylaseSULTERODGSSGBAPLPORuditapes philippinarumGPXGSTGSHUGTNrf2AHRGFPCATkeap1 - buy1RNA interference - RNA تداخل کنندهRNAi - RNA سرکوبگر،RNA مداخلهگر، RNA خاموش کنندهROS - ROSBenzo(a)pyrene - بنزو (a) پیرنهSOD - سدsulfotransferase - سولفوترانسفرازSuperoxide dismutase - سوکسوکس دیسموتازNF-E2-related factor 2 - عامل NF-E2 2Lipid peroxidase - لیپید پراکسیدازmalondialdehyde - مالون دی آلدهیدphosphate buffered solution - محلول بافر فسفاتPolycyclic aromatic hydrocarbons - هیدروکربن آروماتیک چندحلقهایPAHs - هیدروکربن های آروماتیک چند حلقه ایgreen fluorescent protein - پروتئین فلورسنت سبزCatalase - کاتالازGlutathione - گلوتاتیونglutathione S-transferase - گلوتاتیون S-ترانسفرازoxidized glutathione - گلوتاتیون اکسید شدهglutathione reductase - گلوتاتیون ردوکتازglutathione peroxidase - گلوتاتیون پراکسیدازReactive oxygen species - گونههای فعال اکسیژنaromatic hydrocarbon receptor - گیرنده هیدروکربن معطر
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم آبزیان
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The NF-E2-related factor 2 (Nrf2) is a master regulator of cellular responses against environmental stresses. In this study we cloned the full-length cDNAs of the RpNrf2 encompassed 2823 bp from the clam Ruditapes philippinarum (R. philippinarum). Sequences alignment and phylogenetic analysis showed that Nrf2 was highly specific in the clams. RpNrf2 expression was detected in gill, digestive gland, mantle and adductor, which the highest transcription level was observed in gill and digestive gland. The gene expressions of RpNrf2, Kelch-like-ECH-associated Protein 1 (Keap1), Cul3-based E3 Ubiquitin Ligase (E3), Glutathione S-transferase (GST-pi), Superoxide Dismutase (SOD), Catalase (CAT) and Glutathione Peroxidase (GPx) in digestive gland was evaluated by real-time PCR after being exposed to benzo(a)pyrene (BaP) (0.25, 1and 4â¯Î¼g/L) for 15 days, which showed that the expression of Nrf2 significantly increased at day 1 and day 6 after exposure (pâ¯<â¯0.05), and there was a negative relationship between the mRNA levels of Nrf2 and Keap1 that indicates the enhancement of Keap1 expression stimulating Nrf2 degradation. RNA interference experiments were conducted to examine the expression profiles of RpNrf2, antioxidant and detoxification genes (GST-pi, Cu/Zn-SOD, CAT and GPx) and Lipid Peroxidase (LPO) level in digestive gland exposed to BaP. The results showed that the mRNA level of Nrf2 was significantly decreased by 63.2%, and the changes of antioxidant and detoxification genes expression were consistent with the Nrf2 gene suggesting that Nrf2 is required for the induction of antioxidant and detoxification genes. Besides, the LPO levels expressed by malondialdehyde (MDA) contents were significant higher compared with the control group at 72â¯h post dsRNA-Nrf2 injection. In conclusion, our data demonstrated that Keap1 can sense nucleophilic or oxidative stress factors to regulate the Nrf2 signaling pathway together with E3 and Nrf2 signaling pathway plays an important role in modulating gene expression of antioxidant enzymes in bivalve mollusks.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fish & Shellfish Immunology - Volume 80, September 2018, Pages 325-334
Journal: Fish & Shellfish Immunology - Volume 80, September 2018, Pages 325-334
نویسندگان
Hongdan Wang, Luqing Pan, Lingjun Si, Jingjing Miao,