کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8554143 | 1562700 | 2017 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Down-regulation of the expression of alcohol dehydrogenase 4 and CYP2E1 by the combination of α-endosulfan and dioxin in HepaRG human cells
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کلمات کلیدی
ADH4PXRAHROCPARNTTCDDCyPADHALD - آدرنولکودیستروفیOrganochlorine pesticide - آفت کش های آلی کلرورPersistent organic pollutant - آلاینده های پایدار آلیAlcohol dehydrogenase - الکل دهیدروژنازalcohol liver disease - بیماری الکل کبدیCytochrome P450 - سیتوکروم پی۴۵۰AhR nuclear translocator - فرستنده هسته ای AhRCAR - ماشینpop - پاپLiver - کبدaryl hydrocarbon receptor - گیرنده آرویل هیدروکربنconstitutive androstane receptor - گیرنده آندروستان پایدارEstrogen receptor - گیرنده استروژنPregnane X receptor - گیرنده پیش گران X
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Down-regulation of the expression of alcohol dehydrogenase 4 and CYP2E1 by the combination of α-endosulfan and dioxin in HepaRG human cells Down-regulation of the expression of alcohol dehydrogenase 4 and CYP2E1 by the combination of α-endosulfan and dioxin in HepaRG human cells](/preview/png/8554143.png)
چکیده انگلیسی
Pesticides and other persistent organic pollutants are considered as risk factors for liver diseases. We treated the human hepatic cell line HepaRG with both 2,3,7,8 tetrachlorodibenzo-p-dioxin (TCDD) and the organochlorine pesticide, α-endosulfan, to evaluate their combined impact on the expression of hepatic genes involved in alcohol metabolism. We show that the combination of the two pollutants (25 nM TCDD and 10 μM α-endosulfan) led to marked decreases in the amounts of both the mRNA (up to 90%) and protein (up to 60%) of ADH4 and CYP2E1. Similar results were obtained following 24 h or 8 days of treatment with lower concentrations of these pollutants. Experiments with siRNA and AHR agonists and antagonist demonstrated that the genomic AHR/ARNT pathway is necessary for the dioxin effect. The PXR, CAR and estrogen receptor alpha transcription factors were not modulators of the effects of α-endosulfan, as assessed by siRNA transfection. In another human hepatic cell line, HepG2, TCDD decreased the expression of ADH4 and CYP2E1 mRNAs whereas α-endosulfan had no effect on these genes. Our results demonstrate that exposure to a mixture of pollutants may deregulate hepatic metabolism.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology in Vitro - Volume 45, Part 3, December 2017, Pages 309-317
Journal: Toxicology in Vitro - Volume 45, Part 3, December 2017, Pages 309-317
نویسندگان
Eléonore A. Attignon, Emilie Distel, Béatrice Le-Grand, Alix F. Leblanc, Robert Barouki, Eliandre de Oliveira, Martine Aggerbeck, Etienne B. Blanc,