کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8841525 | 1615024 | 2018 | 32 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-494-3p modulates the progression of in vitro and in vivo Parkinson's disease models by targeting SIRT3
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کلمات کلیدی
snRNAMPTPIL-1βDCFH-DAHRPGAPDH1-methyl-4-phenylpyridiniumSirt3miRNAsTRPM7Sirtuin 3transient receptor potential melastatin 71-methyl-4-phenyl-1,2,3,6-tetrahydropyridine - 1-methyl-4-phenyl-1،2،3،6-tetrahydropyridine2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetateDMSO - DMSOMPP+ - MPP +MTT - MTTsmall nuclear RNA - RNA هسته ای کوچکROS - ROSSNpc - SNPCSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدInterleukin-1β - اینترلوکین-1βParkinson’s disease - بیماری پارکینسونELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاsubstantia nigra pars compacta - توده سیاه پارس متراکمtumor necrosis factor-α - تومور نکروز عامل αDopaminergic - دوپامینرژیکdimethylformamide - دی متیل فرمالیدmicroRNAs - ریز آرانایSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازTNF-α - فاکتور نکروز توموری آلفاlactate dehydrogenase - لاکتات دهیدروژناز LDH - لاکتات دهیدروژناز به صورت مختصر شده LDH Horseradish peroxidase - پراکسیداز هوررادیشglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علم عصب شناسی
علوم اعصاب (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Parkinson's disease (PD) is the second most prevalent chronic and progressive neurodegenerative disease. Plenty of miRNAs have been demonstrated to participate in the pathogenesis of PD. However, the detailed roles of miR-494-3p and underlying mechanisms involved in PD progression remain to be explored. In the present study, we found that miR-494-3p expression was increased and sirtuin 3 (SIRT3) expression was decreased in SH-SY5Y cells following 1-Methyl-4-phenylpyridinium (MPP+) treatment. Loss-of-function showed that miR-494-3p inhibition promoted cell viability and superoxide dismutase (SOD) activity, and suppressed apoptotic rate, caspase-3 activity, lactate dehydrogenase (LDH) activity, tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) expressions, and reactive oxygen species (ROS) generation in MPP+-induced SH-SY5Y cells. Moreover, SIRT3 was identified as a target of miR-494-3p and miR-494-3p negatively regulated SIRT3 expression in SH-SY5Y cells. Additionally, up-regulation of miR-494-3p suppressed SIRT3 expression and exacerbated motor impairment in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model. In conclusion, miR-494-3p inhibition exerted a neuroprotective role in MPP+-induced PD by targeting SIRT3, providing a possible therapeutic strategy for PD patients.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neuroscience Letters - Volume 675, 14 May 2018, Pages 23-30
Journal: Neuroscience Letters - Volume 675, 14 May 2018, Pages 23-30
نویسندگان
Lijiao Geng, Tao Zhang, Wei Liu, Yong Chen,