کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9886592 | 1537838 | 2005 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Statins potentiate the IFN-γ-induced upregulation of group IIA phospholipase A2 in human aortic smooth muscle cells and HepG2 hepatoma cells
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کلمات کلیدی
GGTIHASMCHMG-CoATcdBcPLA2sPLA2-IIAIFN-γDRBFCSIL-1βPSIERKGAPDHPBSIL-6GGPP5,6-dichloro-1-β-d-ribofuranosylbenzimidazoleFTiFPPHDLRT-PCRJanus kinase - کیناز جانوس 3-hydroxy-3-methylglutaryl-coenzyme A - 3-هیدروکسی-3-methylglutaryl-coenzyme AC/EBP-β - C / EBP-βClostridium difficile toxin B - Clostridium difficile B سم زداییNFκB - NFKBSTAT - آمارenzyme linked immunosorbent assay - آنزیم تست ایمونوسیورسانس مرتبط استStatin - استاتینSecretory phospholipase A2 - اسید فسفولیپاز A2inflammation - التهاب( توروم) interferon-γ - اینترفرون-γinterleukin-6 - اینترلوکین ۶Interleukin-1β - اینترلوکین-1βELISA - تست الیزاtumor necrosis factor-α - تومور نکروز عامل αfetal calf serum - سرم گوساله جنینhuman aortic smooth muscle cells - سلول های عضلانی صاف آئورت انسانHepG2 cells - سلولهای HepG2farnesyl pyrophosphate - فارسیل پیرو فسفاتTNF-α - فاکتور نکروز توموری آلفاnuclear factor-κB - فاکتور هسته ای κBcytosolic phospholipase A2 - فسفولیپاز A2 سیتوزولHigh-density lipoproteins - لیپوپروتئینهای با چگالی بالاSignal transducer and activator of transcription - مبدل سیگنال و فعال کننده رونویسیMEK - مجاهدین خلقPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHMG-CoA reductase inhibitor - مهار کننده HMG-CoA ردوکتازProteasome inhibitor - مهار کننده پروتئازومFarnesyl transferase inhibitor - مهارکننده Farnesyl Transferasereverse transcription polymerase chain reaction - واکنش زنجیره ای پلیمراز رونویسی معکوسJAK - چگونهgeranylgeranyl pyrophosphate - ژرینیل گرانیل پیرو فسفاتextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The present study shows that the incubation of human aortic smooth muscle cells (HASMC) and HepG2 cells with atorvastatin and mevastatin as HMG-CoA reductase inhibitors potentiated the interferon-γ (INF-γ)-induced group IIA phospholipase A2 (sPLA2-IIA) expression in a dose- and time-dependent manner. The effect of statins on sPLA2-IIA expression was reduced by mevalonate, farnesyl pyrophosphate and geranylgeranyl pyrophosphate. Inversely, inhibitors of the farnesyl transferase and geranylgeranyl transferase-I mimicked the effects of statins. Clostridium difficile toxin B (TcdB), Y-27632 and H-1152, functioning as inhibitors of Rho proteins and Rho-associated kinase, also augmented the sPLA2-IIA expression in combination with IFN-γ. The same effects were observed when inhibitors of mitogen-activated/extracellular response protein kinase kinase (MEK), PD98059 or U0126 were used. Further, the Janus kinase-2 (Jak2)-specific inhibitor, AG-490 and inhibitors of nuclear factor-κB (NFκB) abrogated the sPLA2-IIA elevating effects of statins, TcdB and PD98059 in the presence of IFN-γ. This cytokine alone increased the NFκB p65 and CAAT-enhancer-binding protein-β (C/EBP-β) activity in HASMC nuclear extract, but only C/EBP-β was further augmented when the cells were incubated in addition to IFN-γ with atorvastatin, H-1152, PD98059 or U0126. Moreover, after the incubation of cells with atorvastatin and IFN-γ the stability of sPLA-2IIA mRNA significantly increased in comparison to those after incubation with IFN-γ alone. In conclusion, the obtained data suggest that (i) the expression of sPLA2-IIA is negatively regulated by RhoA/Rho-associated kinase and MEK/ERK signaling pathways and (ii) statins, because of their ability to down-regulate these pathways, can potentiate the IFN-γ-induced sPLA2-II expression at transcriptional and post-transcriptional levels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1733, Issues 2â3, 15 April 2005, Pages 157-171
Journal: Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids - Volume 1733, Issues 2â3, 15 April 2005, Pages 157-171
نویسندگان
Mario Menschikowski, Albert Hagelgans, Ben Heyne, Ute Hempel, Volker Neumeister, Peter Goez, Werner Jaross, Gabriele Siegert,