کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5510475 | 1539128 | 2017 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cloning and expression characterization of peroxisome proliferator-activated receptors (PPARs) with their agonists, dietary lipids, and ambient salinity in rabbitfish Siganus canaliculatus
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کلمات کلیدی
activation function 1SREBPRXRHNF4αLXRSREAF1Retinoid X receptorAF2LC-PUFAlinoleicALAELOVLSterol regulatory element binding proteinsORFEtOHPPAR response elementsEPADulbecco's Modified Eagle Medium: Nutrient Mixture F-12FBSEicosapentaenoic acid (20:5n-3)PPREsDBDLC-PUFA biosynthesisLBDarachidonic acid (20:4n-6)2-Bromopalmitate - 2-بروموپالمیتاتcDNA - cDNADMEM/F12 - DMEM / F12DMSO - DMSOPPARs - PPAR هاAgonist - آگونیستEthanol - اتانولdocosahexaenoic acid (22:6n-3) - اسید داکوزاگزائنیک (22: 6n-3)complementary deoxyribonucleic acid - اسید دز اکسید ریبونوکلئیک مکملPolyunsaturated fatty acids - اسید چرب اشباع نشدهPUFA - اسید چرب چند غیراشباعLong-chain polyunsaturated fatty acids - اسیدهای چرب اشباع نشده چای زنجیره ایFAD - بدactivation function 2 - تابع فعال سازی 2DNA-Binding Domain - دامنه اتصال DNAligand binding domain - دامنه اتصال لیگاندARA - در حال حاضرDHA - دوکوساهگزائنوئیک اسیدdimethyl sulphoxide - دی متیل سولفوکسیدFish oil - روغن ماهیVegetable oils - روغن های گیاهیfetal bovine serum - سرم جنین گاوsterol regulatory element - عنصر نظارتی استرولOpen reading frames - فریم های خواندن را باز کنیدFenofibrate - فنوفیبراتpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازliver X receptor - کبد X گیرندهPeroxisome proliferator-activated receptors - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cloning and expression characterization of peroxisome proliferator-activated receptors (PPARs) with their agonists, dietary lipids, and ambient salinity in rabbitfish Siganus canaliculatus Cloning and expression characterization of peroxisome proliferator-activated receptors (PPARs) with their agonists, dietary lipids, and ambient salinity in rabbitfish Siganus canaliculatus](/preview/png/5510475.png)
چکیده انگلیسی
Rabbitfish Siganus canaliculatus is the first marine teleost reported to have the ability of biosynthesizing C20-22 long-chain polyunsaturated fatty acids (LC-PUFA) from C18 precursors, and thus provides a model for studying the regulatory mechanisms of LC-PUFA biosynthesis in teleosts. To investigate the possible roles of peroxisome proliferator-activated receptors (PPARs), critical transcription factors involved in the regulation of lipid metabolism, in the regulation of LC-PUFA biosynthesis in rabbitfish, the PPAR genes were cloned and their expression characterization with PPAR agonists, dietary lipid resource, and ambient salinity were examined. Three cDNA sequences respectively encoding 477, 516 and 519 amino acids of PPARα, PPARβ, and PPARγ isoforms were obtained. PPARα exhibited a wide tissue expression with its highest levels in the heart and brain; PPARβ was predominantly expressed in the gills, while PPARγ was highly expressed in the intestine and gills. In rabbitfish primary hepatocytes, both the PPAR agonists 2-bromopalmitate (2-Bro) and fenofibrate (FF) increased the expression of PPARγ, SREBP1c and Elovl5, whereas FF depressed the expression of Î6/Î5 Fad. Moreover, a higher hepatic PPARβ expression was observed in fish fed diets with vegetable oils (VO) than that with fish oil (FO), in the former the expression of PPARα, PPARβ, and PPARγ were increased at the low ambient salinity (10 ppt), where an increasing expression of Î5/Î6 Fad, Î4 Fad and Elovl5 genes was previously reported. These results suggest that PPARs might be involved in the upregulation of LC-PUFA biosynthesis with dietary VO and low ambient salinity in rabbitfish.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 206, April 2017, Pages 54-64
Journal: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology - Volume 206, April 2017, Pages 54-64
نویسندگان
Cuihong You, Danli Jiang, Qinghao Zhang, Dizhi Xie, Shuqi Wang, Yewei Dong, Yuanyou Li,