Identification and characterization of reactive metabolites in myristicin-mediated mechanism-based inhibition of CYP1A2

It is identified that myristicin is a mechanism-based inhibitor of CYP1A2 through CYP cocktail screening, IC50 shift calculation and GSH capturing assays in HLMs. And the reactive metabolites were identified as a quinone and its tautomer through detailed analysis the cleavage processes of metabolites and GSH-myristicin adduct in phase I metabolism assays using UPLC-MS2.