کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5848078 | 1561632 | 2014 | 15 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of hydroxylated resveratrol analogs on oxidative stress and cancer cells death in human acute T cell leukemia cell line
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کلمات کلیدی
CATGSSGSK-Mel-28DETAPACDDTCResveratrol analogsPNAAsPC-1GPXGSTGSHTBARS2′7′-dichlorofluoresceinRPMIDcfCDNBMDAHHRAMnSODFBSDMEMPBSJC-11-chloro-2,4-dinitrobenzene - 1-کلرو-2،4-دینیتروبنزن2′,7′-dichlorodihydrofluorescein diacetate - 2 '، 7'-dichlorodihydrofluorescein diacetateDMSO - DMSOH2DCFDA - H2DCFD بهKCN - IP هاROS - ROSEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدThiobarbituric acid - اسید تیوباربیتوریکDiethylenetriaminepentaacetic acid - دی اتیلنتریمین پنتا اسیدهای اسیدdiethyldithiocarbamate - دیاتیدیدیتیو کرباماتDimethylsulfoxide - دیمتیل سولفواکسیدResveratrol - رسوراترولfetal bovine serum - سرم جنین گاوSuperoxide dismutase - سوکسوکس دیسموتازPhosphate buffered saline - فسفات بافر شورmalondialdehyde - مالون دی آلدهیدDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoRoswell Park Memorial Institute medium - موسسه خاطرات Roswell Park mediumpotassium cyanide - پتاسیم سیانیدCatalase - کاتالازCaspase-3 - کاسپاز ۳ Caspase-8 - کاسپاز-8Caspase-9 - کاسپاز-9reduced glutathione - کاهش گلوتاتیونglutathione-S-transferase - گلوتاتیون S-ترانسفرازoxidized glutathione - گلوتاتیون اکسید شدهglutathione reductase - گلوتاتیون ردوکتازglutathione peroxidase - گلوتاتیون پراکسیدازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Resveratrol and its higher hydroxylated analogs have been reported to possess a variety of biological properties including antioxidant as well as prooxidant effects. The antioxidant properties are assumed to enable these compounds to protect cells from oxidative damage, however prooxidant activity are held likely to be responsible for their cytotoxic or pro-apoptotic effects. In present study the effects of resveratrol (Res) and its three derivatives: 3,3â²,4,4â²-tetrahydroxy-trans-stilbene (M6), 3,4,4â²,5-tetrahydroxy-trans-stilbene (M8) and 3,3â²,4,4â²,5,5â²-hexahydroxy-trans-stilbene (M12) were investigated on T cell leukemia Jurkat cells. The tested compounds have cytotoxic activity against cancer cells and IC50 values obtained in the Alamar blue assay were: 58.4 μM, 48.1 μM, 33.4 μM for and 13.8 μM for Res, M6, M8, M12, respectively. Furthermore, we also observed an increased activity of caspase 3 and 9, with significantly higher values in cells incubated with M8 and M12 than Res and M6. Cell death was accompanied by loss of mitochondrial potential, oxidative stress, decrease of glutathione level as well as loss of both mRNA expression and activity of superoxide dismutase (MnSOD). Cytotoxic activity may be connected with the formation of short-living prooxidative metabolites as compounds M8 and M12 were very instable in incubation medium. In conclusion, we elucidated the mechanisms responsible for cytotoxicity of hydroxylated resveratrol analogs in leukemia cells which may also apply to other polyphenols.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 209, 25 February 2014, Pages 96-110
Journal: Chemico-Biological Interactions - Volume 209, 25 February 2014, Pages 96-110
نویسندگان
Malgorzata Kucinska, Hanna Piotrowska, MichaÅ W. Luczak, Justyna Mikula-Pietrasik, Krzysztof Ksiazek, Milena Wozniak, Marcin Wierzchowski, Jaroslaw Dudka, Walter Jäger, Marek Murias,