کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9485689 | 1329283 | 2005 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effects of light intensities on antioxidant enzymes and malondialdehyde content during short-term acclimatization on micropropagated Phalaenopsis plantlet
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کلمات کلیدی
GSTGSSGMDHARPPFDNBTDTNBDHARCDNBAPXPMSFGSHTBAPhalaenopsisCATG-PODMDAPVPPoly Vinyl Pyrrolidone1-chloro-2,4-dinitrobenzene - 1-کلرو-2،4-دینیتروبنزن2-Thiobarbituric acid - 2-تیوباربیتوریک اسید5,5′-dithiobis (2-nitrobenzoic acid) - 5،5'-dithiobis (2-nitrobenzoic acid)Fv/Fm - Fv / FmROS - ROSascorbate oxidase - آسکوربات اکسیدازAntioxidative enzymes - آنزیم های آنتی اکسیداتیوLOX - اکسیژن مایعhigh light - برجستهMdha - بزرگترnitro blue tetrazolium - تترازولیم نیترو آبیDHA - دوکوساهگزائنوئیک اسیدSOD - سدSuperoxide dismutase - سوکسوکس دیسموتازLight intensity - شدت نورphotosynthetic photon flux density - فتوسنتز تراکم شار فوتونphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدlipoxygenase - لیپواکسیژنازmalondialdehyde - مالون دی آلدهیدlow light - نور کمascorbate peroxidase - پراکسیداز آسکورباتیLipid peroxidation - پراکسیداسیون لیپیدCatalase - کاتالازPhotosynthetic efficiency - کارایی فتوسنتزیreduced glutathione - کاهش گلوتاتیونglutathione S transferase - گلوتاتیون S transferaseoxidized glutathione - گلوتاتیون اکسید شدهglutathione reductase - گلوتاتیون ردوکتازguaiacol peroxidase - گوایاکول پراکسیدازReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
بوم شناسی، تکامل، رفتار و سامانه شناسی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We investigated the effects of light stress on the activities of antioxidant enzymes, such as superoxide dismutase (SOD), dehydro ascorbate reductase (DHAR), monodehydro ascorbate reductase (MDHAR), ascorbate oxidase (AO), glutathione reductase (GR), guaiacol peroxidase (G-POD), catalase (CAT), glutathione S transferase (GST), lipoxygenase (LOX), lipid peroxidation (LP), leaf protein content and photosynthetic efficiency (Fv/Fm) in order to evaluate their role during acclimatization in Phalaenopsis orchids. Six months old in vitro grown plantlets were exposed to low light (LL-60 μmol mâ2 sâ1), intermediate light (IL-160 μmol mâ2 sâ1) and high light (HL-300 μmol mâ2 sâ1) photosynthetic photon flux density (PPFD), respectively under controlled condition. Plantlets exposed to HL intensity had lower level of Fv/Fm ratio than the LL grown plantlets during acclimatization. Regarding antioxidants enzymes, SOD activity increased in leaves with increasing light intensity but light stress had no significant effect in roots. DHAR and MDHAR activities increased in LL and IL but decreased at HL. The CAT activity increased in both leaves and roots with increasing light intensity. While G-POD activity increased in roots, POD activity was not detected in leaves. No significant change in GR activity has been found at IL and HL, though it decreased significantly at LL compared to in vitro grown plantlets. There was an increase in AO activity in leaves of about 50% at HL compared to in vitro grown plantlets, whereas no changes in roots were observed. GST activity showed pronounced stimulation in both leaves and roots of the plantlets exposed to HL compared to in vitro grown ones. Total leaf protein content increased in light stressed plantlets compared to in vitro grown plantlets. LP and LOX increased during light stress compared to in vitro grown plantlets suggesting that LOX mediated lipid peroxidation contributed to the oxidative damage occurring in the present study. These results suggest that increase in enzyme activities were an adaptive response of the plantlets to higher amounts of reactive oxygen species (ROS) generated during acclimatization under light stress.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Environmental and Experimental Botany - Volume 54, Issue 2, September 2005, Pages 109-120
Journal: Environmental and Experimental Botany - Volume 54, Issue 2, September 2005, Pages 109-120
نویسندگان
Mohammad Babar Ali, Eun-Joo Hahn, Kee-Yoeup Paek,