کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
6493402 | 1418055 | 2018 | 30 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Photochemoprotective effect of a fraction of a partially purified extract of Byrsonima crassifolia leaves against UVB-induced oxidative stress in fibroblasts and hairless mice
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کلمات کلیدی
SDSCATUVBEGTAGSHEGCGBCFFBSDMEMOPTPBSPMSFMMP(+)-catechin - (+) - کاتچینMPO - DFODMSO - DMSODulbecco's modified Eagle Medium - Eagle Medium اصلاح شده Dulbeccoo-Phthalaldehyde - o-فتالالدئیدROS - ROSEDTA - اتیلن دی آمین تترا استیک اسید ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیست (2-aminoethylether) -N، N، N '، N'-tetraacetic acidethylenediamine tetraacetic acid - اسید اتیلنیدین تتراستیکUltraviolet radiation - اشعه ماوراء بنفشultraviolet B radiation - اشعه ماوراء بنفش Bepigallocatechin gallate - اپی گالوستاچین گالاتinterleukin - اینترلوکینtumor necrosis factor alpha - تومور نکروز عامل آلفاDimethyl sulfoxide - دیمتیل سولفواکسیدsodium dodecyl sulfate - سدیم دودسیل سولفاتfetal bovine serum - سرم جنین گاوCytokines - سیتوکین هاTNF-α - فاکتور نکروز توموری آلفاPhosphate buffered saline - فسفات بافر شورAntioxidant activity - فعالیت آنتیاکسیدانیphenylmethylsulfonyl fluoride - فنیل متیل سولفونیل فلورایدMetalloproteinase - متالوپروتئیناز myeloperoxidase - میلوپراکسیداز Catechin - کاتچین، کتیچینreduced glutathione - کاهش گلوتاتیونGallic acid - گالیک اسیدReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Ultraviolet B (UVB) irradiation increases the risk of various skin disorders, leading to inflammation and oxidative stress and thereby increasing the risk of skin photoaging and carcinogenesis. The use of photochemoprotectors such as natural products with antioxidant and anti-inflammatory properties represents a strategy for preventing UVB-induced skin damage. We investigated the photochemoprotective effect of a fraction of a partially purified extract of Byrsonima crassifolia leaves (BCF) on fibroblasts and hairless mice exposed to UVB radiation. The mixture of phenolic compounds in BCF prevented the decrease in reduced glutathione (GSH) levels in fibroblast cultures induced by UVB radiation more than some of their individual standards ((+)-catechin (CAT), epigallocatechin gallate and quercetin 3-O-β-d-glucopyranoside). Prepared gel formulations increased skin antioxidant activity, and BCF components and the CAT standard were retained in the HRS/J hairless mice epidermis 2 h after application. Topical treatment with the BCF or CAT formulations (1%) significantly reduced the decrease in GSH levels and decreased myeloperoxidase activity and the secretion of pro-inflammatory cytokines IL-1β and IL-6 induced by UVB radiation (P < 0.05), indicating that both BCF and CAT had anti-inflammatory effects. BCF inhibited UVB-induced metalloproteinase (MMP)-9 secretion/activity, whereas CAT had no effect on MMP-9 activity in the skin of treated animals. These results therefore suggest that BCF can be used as a photochemoprotective agent and antioxidant in the prevention/treatment of inflammation and oxidative stress of the skin induced by UVB radiation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 178, January 2018, Pages 53-60
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 178, January 2018, Pages 53-60
نویسندگان
Rebeca Oliveira de Souza, Geórgia de Assis Dias Alves, Ana Luiza Scarano Aguillera, Hervé Rogez, Maria José Vieira Fonseca,