کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7279136 | 1473895 | 2018 | 52 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Triptolide up-regulates metabotropic glutamate receptor 5 to inhibit microglia activation in the lipopolysaccharide-induced model of Parkinson's disease
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
CREBDHPGPLCPKCMPEPionized calcium binding adaptor moleculeiNOSIBAERKCHPGGAPDH(RS)-2-chloro-5-hydroxyphenylglycineMTEPTriptolideJnkmGluCOXLPST10TNF(S)-3,5-dihydroxyphenylglycine - (S) -3،5-دی هیدروکسی فنیل گلیسین2-methyl-6-(phenylethynyl)-pyridine - 2-متیل-6- (فنیلئتینیل) -پیریدینc-Jun N-terminal kinase - C-Jun N-terminal kinaseMAPKs - MAPK هاcyclooxygenase - آنزیم سیکلواکسیژنازAkt - آکتinflammation - التهاب( توروم) interleukin - اینترلوکینBAPTA-AM - بیایپیتیای-AMParkinson’s disease - بیماری پارکینسونsubstantia nigra - توده سیاهtyrosine hydroxylase - تیروزین هیدروکسیلازinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییnuclear factor - عامل هسته ایtumor necrosis factor - فاکتور نکروز تومورphospholipase C - فسفولیپاز Clipopolysaccharide - لیپوپلی ساکاریدMicroglia - میکروگلیاهاNitric oxide - نیتریک اکسیدcyclic AMP response element-binding protein - پروتئین اتصال دهنده عنصر Response Cyclic AMPprotein kinase B - پروتئین کیناز BProtein kinase C - پروتئین کیناز سیExtracellular signal-regulated protein kinase - پروتئین کیناز کنترل شده سیگنال غیر سلولیmitogen-activated protein kinases - کیناز پروتئین فعال Mitogenglyceraldehyde 3-phosphate dehydrogenase - گلیسرولیدید 3-فسفات دهیدروژنازmetabotropic glutamate receptor 5 - گیرنده گلوتامات metabotropic 5Metabotropic glutamate receptor - گیرنده گلوتامات متابوتروپیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
ایمنی شناسی و میکروب شناسی
ایمونولوژی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Metabotropic glutamate receptor (mGlu)5 regulates microglia activation, which contributes to inflammation. However, the role of mGlu5 in neuroinflammation associated with Parkinson's disease (PD) remains unclear. Triptolide (T10) exerts potent immunosuppressive and anti-inflammatory effects and protects neurons by inhibiting microglia activation. In this study, we investigated the role of mGlu5 in the anti-inflammatory effect of T10 in a lipopolysaccharide (LPS)-induced PD model. In cultured BV2 cells and primary microglia, blocking mGlu5 activity or knocking down its expression abolished T10-inhibited release of proinflammatory cytokines induced by LPS. Moreover, T10 up-regulated mGlu5 expression decreased by LPS through enhancing mRNA expression and protein stability. T10 also reversed the reduction in mGlu5 membrane localization and modulated receptor-mediated mitogen-activated protein kinase activity induced by LPS. Pharmacological inhibition of signaling molecules increased nitric oxide level and inducible nitric oxide synthase (iNOS), tumor necrosis factor-α, and interleukin (IL)-1β and -6 transcript levels that were downregulated by treatment with T10. Consistent with these in vitro findings, blocking mGlu5 attenuated the anti-inflammatory effects of T10 in an LPS-induced PD model and blocked the decreases in the number and morphology of ionized calcium binding adaptor molecule 1-positive microglia and LPS-induced iNOS protein expression caused by T10 treatment. Besides, mGlu5 mediated the effect of T10 on microglia-induced astrocyte activation in vitro and in vivo. The findings provide evidence for a novel mechanism by which mGlu5 regulates T10-inhibited microglia activation via modulating protein expression of the receptor and its intracellular signaling. The study might contribute to the biological effects of Chinese herbs as an approach for protecting against neurotoxicity in PD.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Brain, Behavior, and Immunity - Volume 71, July 2018, Pages 93-107
Journal: Brain, Behavior, and Immunity - Volume 71, July 2018, Pages 93-107
نویسندگان
Yi-Ying Huang, Qian Zhang, Jian-Nan Zhang, Ya-Nan Zhang, Li Gu, Hui-Min Yang, Ning Xia, Xiao-Min Wang, Hong Zhang,