کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8551242 | 1562107 | 2018 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A human relevance investigation of PPARα-mediated key events in the hepatocarcinogenic mode of action of propaquizafop in rats
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کلمات کلیدی
Human relevance frameworkacyl-CoA oxidaseHRFMOAIPCsPPARαMRMGSSGACOWOEGSHPRODCATCyPmRNAPBSIARCDNA - DNA یا اسید دزوکسی ریبونوکلئیکGSH-Px - GSH-PXH&E - H & EPeroxisome proliferators - proliferators پروکسیومInternational Agency for Research on Cancer - آژانس بین المللی تحقیقات سرطانUnited States Environmental Protection Agency - آژانس حفاظت از محیط زیست ایالات متحدهdeoxyribonucleic acid - اسید deoxyribonucleicEnzyme induction - القاء آنزیمstandard deviation - انحراف معیارInternational Programme on Chemical Safety - برنامه بین المللی ایمنی مواد شیمیاییBrdU - بروموداکسی اوریدینbromodeoxyuridine - برومودسوویریدینMode of action - حالت عملmessenger ribonucleic acid - رسوب ریبونوکلئیک اسیدRIN - رینWorld Health Organization - سازمان بهداشت جهانیSOD - سدCarcinogenicity - سرطان زاییSuperoxide dismutase - سوکسوکس دیسموتازCytochrome P450 - سیتوکروم پی۴۵۰RNA Integrity Number - شماره یکپارچه RNAPhosphate buffered saline - فسفات بافر شورRats - موش صحراییhematoxylin-eosin - هماتوکسیلین ائوزینbody weight - وزن بدنWeight of evidence - وزن شواهدCatalase - کاتالازreduced glutathione - کاهش گلوتاتیونLiver - کبدWHO - کهUSEPA - گامglutathione peroxidase - گلوتاتیون پراکسیدازperoxisome proliferator-activated receptor α - گیرنده پروتئینی فعال پروکسایزوم α
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Propaquizafop is an herbicide with demonstrated hepatocarcinogenic activity in rodents. A rodent-specific mode of action (MOA) in the liver via activation of peroxisome proliferator-activated receptor α (PPARα) has been postulated based on existing data. Experience with PPARα-inducing pharmaceuticals indicates a lack of human relevance of this MOA. The objective of the present investigation was to evaluate the dependency of early key events leading to liver tumors on PPARα activation in wildtype (WT) compared to PPARα-knockout (KO) rats following 2 weeks exposure to 75, 500 and 1000â¯ppm propaquizafop in the diet. In WT rats, both WY-14643 (50â¯mg/kgâ¯bw/day) and propaquizafop (dose-dependently) induced marked increases in liver weights, correlating with liver enlargement and hepatocellular hypertrophy, along with increased CYP4A and acyl-CoA oxidase mRNA expression and enzyme activities versus controls, while in KO rats liver weight was mildly increased only at the high dose with minimal microscopic correlates and without any changes in liver peroxisomal or CYP4A activities. In addition, BrdU labeling resulted in higher numbers and density of positive hepatocytes versus controls in WT but not in KO rats, indicating increased mitotic activity and cell proliferation only in WT rats, thus confirming the PPARα-dependency of the biochemical and histological changes in the liver. Based on an assessment of the results of this investigation, together with existing propaquizafop data according to the MOA-Human Relevance Framework, we conclude that liver tumors observed in rodents after dietary administration of propaquizafop do not pose a relevant health risk to humans.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Regulatory Toxicology and Pharmacology - Volume 95, June 2018, Pages 348-361
Journal: Regulatory Toxicology and Pharmacology - Volume 95, June 2018, Pages 348-361
نویسندگان
Christian Strupp, Werner H. Bomann, François Spézia, Frédéric Gervais, Roy Forster, Lysiane Richert, Pramila Singh,