کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
9001632 | 1118539 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
New class of linoleic acid metabolites biosynthesized by corn and rice lipoxygenases: Suppression of proinflammatory mediator expression via attenuation of MAPK- and Akt-, but not PPARγ-, dependent pathways in stimulated macrophages
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کلمات کلیدی
TGFRT-PCRERKCLACOX-2HRPGAPDHiNOSNSAIDPPARIκBAP-1TLRFBSGM-CSF13S-HODEPBSTNFLPSIL-6DMEMCOXInhibitory rate15d-PGJ2Jnk3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromidec-Jun N-terminal kinase - C-Jun N-terminal kinaseDMSO - DMSODulbecco's modified Eagle Medium - Eagle Medium اصلاح شده DulbeccoMAPK - MAPKMTT - MTTNFκB - NFKBNO2− - NO2-cyclooxygenase - آنزیم سیکلواکسیژنازLinoleic acid - اسید لینولئیکconjugated linoleic acid - اسید لینولئیک کونژوگهstandard deviation - انحراف معیارLOX - اکسیژن مایعinterferon - اینترفرونIFN - اینترفرون هاinterleukin - اینترلوکینtransforming growth factor - تبدیل فاکتور رشدToll-like receptor - تیالآرNonsteroidal anti-inflammatory drugs - داروهای ضد التهابی غیر استروئیدیDimethylsulfoxide - دیمتیل سولفواکسیدCell viability - زنده ماندن سلولfetal bovine serum - سرم جنین گاوinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییAnti-inflammation - ضد التهابgranulocyte-macrophage colony-stimulating factor - عامل گرانولوسیت-ماکروفاژ colony-stimulating factortumor necrosis factor - فاکتور نکروز تومورnuclear factor-kappaB - فاکتور هسته ای - kappaBlipoxygenase - لیپواکسیژنازlipopolysaccharide - لیپوپلی ساکاریدMacrophage - ماکروفاژ Phosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNitrite - نیتریتNitric oxide - نیتریک اکسیدreverse transcription-polymerase chain reaction - واکنش زنجیره ای رونویسی-پلیمراز معکوسHorseradish peroxidase - پراکسیداز هوررادیشactivator protein-1 - پروتئین فعال کننده-1mitogen-activated protein kinase - پروتئین کیناز فعال با mitogenprostaglandin - پروستاگلاندینهاhigh performance liquid chromatography - کروماتوگرافی مایع با کارایی بالاHPLC - کروماتوگرافی مایعی کاراextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولیglyceraldehyde-3-phosphate dehydrogenase - گلیسرالیدید-3-فسفات دهیدروژنازperoxisome proliferator-activated receptor - گیرنده فعال فعال پروکسیوم
موضوعات مرتبط
علوم پزشکی و سلامت
داروسازی، سم شناسی و علوم دارویی
داروشناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: New class of linoleic acid metabolites biosynthesized by corn and rice lipoxygenases: Suppression of proinflammatory mediator expression via attenuation of MAPK- and Akt-, but not PPARγ-, dependent pathways in stimulated macrophages New class of linoleic acid metabolites biosynthesized by corn and rice lipoxygenases: Suppression of proinflammatory mediator expression via attenuation of MAPK- and Akt-, but not PPARγ-, dependent pathways in stimulated macrophages](/preview/png/9001632.png)
چکیده انگلیسی
In response to endogenous and exogenous stimuli, macrophages are activated to produce a cocktail of proinflammatory and anti-apoptotic mediators, thereby participating in the processes of inflammation-associated oncogenesis. Cereals, including corn and rice, have biological potentials to synthesize self-protective chemicals in order to repel the invasion of microorganisms and insects. We examined the suppressive effects of several fatty acids, including a new class of lipoxygenase metabolites of linoleic acid (LA) found in cereals, namely (±)-9-hydroxy-trans,cis-10,12-octadecadienoic acid (9-HOA from rice), (±)-13-hydroxy-10-oxo-trans-11-octadecenoic acid (13-HOA from corn), and (±)-10-oxo-trans-11-octadecen-13-olide (10-ODO from corn), on lipopolysaccharide (LPS)-induced mRNA expression of proinflammatory mediators in RAW264.7 murine macrophages. Each metabolite exhibited a suppressive activity toward nitrite production than LA, octadeca-9Z,11E-dienoic acid (a conjugated LA), and 13(S)-hydroxyoctadeca-9Z,11E-dienoic acid. LPS-up-regulated mRNA expression of inducible nitric oxide synthase, cyclooxygenase (COX)-2, interleukin-6, and toll-like receptor-2, -4, and -9 was also markedly attenuated without affecting the expression levels of several constitutive genes, including COX-1, as detected by reverse transcription-polymerase chain reactions. In addition, Western blot and luciferase reporter assay results showed that 13-HOA suppressed the phosphorylation of mitogen-activated protein kinases (extracellular signal-regulated kinasel/2, c-Jun N-terminal kinasel/2, p38 mitogen-activated protein kinase), and Akt (Ser473), and also attenuated degradation of inhibitor kappaB, nuclear translocation of nuclear factor kappaB (NFκB), and the transcriptional activities of NFκB and activator protein-1, both of which have essential roles in the transcription of numerous proinflammatory and oncogenic genes. In contrast, 13-HOA did not serve as a ligand for peroxisome proliferator-activated receptor-γ. Based on our findings, we propose that 13-HOA, a functionally novel LA-derivative, is a promising agent for anti-inflammatory and chemopreventive strategies with reasonable molecular mechanisms.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical Pharmacology - Volume 70, Issue 9, 1 November 2005, Pages 1330-1342
Journal: Biochemical Pharmacology - Volume 70, Issue 9, 1 November 2005, Pages 1330-1342
نویسندگان
Akira Murakami, Takashi Nishizawa, Kahori Egawa, Teruo Kawada, Yasushi Nishikawa, Kazuo Uenakai, Hajime Ohigashi,