کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7620385 | 1494498 | 2015 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Peanut traces in packaged food products consumed by allergic individuals: Results of the MIRABEL project
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کلمات کلیدی
CTABLOAELLFADNA - DNA یا اسید دزوکسی ریبونوکلئیکReal-time PCR - PCR به موقعCOV - THELateral flow assay - آزمون جریان جانبیFood allergen - آلرژن غذاییCetyltrimethyl ammonium bromide - استیلتریال آمونیوم برومیدdesoxyribonucleic acid - اسید desoxyribonucleicstandard deviation - انحراف معیارFood analysis - تجزیه و تحلیل مواد غذاییFood composition - ترکیب مواد غذاییELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاLOD یا Limit of detection - حد تشخیصEffective dose - دوز موثرlevel of detection - سطح تشخیصCoefficient of Variation - ضریب تغییرpolymerase chain reaction - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازLowest observed adverse effect level - کمترین میزان بروز ناخواسته مشاهده شده
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In the frame of the French research project MIRABEL, 899 food samples which contained no peanut ingredients according to the labeling list were analyzed for the presence of peanut allergen traces. Samples covered a broad range of products from ten major food categories. In a stepwise procedure, samples were screened using a sensitive lateral flow assay with a limit of detection of 2Â ppm total peanut or 0.5Â ppm peanut protein. Positive as well as suspect samples (139/899) were confirmed by real-time PCR with the same sensitivity. Positives in both approaches were quantified by two different commercial ELISA tests. 1% (9/899) out of all samples did contain measurable peanut DNA and protein traces above the detection limit of the applied methods. Six samples had a content of total peanut protein <5Â mg/kg, two samples contained between 8 and 10Â mg/kg and one sample a maximum of about 20Â mg/kg. An excellent correlation was found between Ct-values obtained by PCR and ppm peanut calculated by ELISA. It is concluded that, in the light of future thresholds for labeling of relevant allergens, the methods used for peanut detection in this study are able to detect contaminations as low as 2Â ppm.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Composition and Analysis - Volume 44, December 2015, Pages 196-204
Journal: Journal of Food Composition and Analysis - Volume 44, December 2015, Pages 196-204
نویسندگان
Jutta Zagon, Joerg Dittmer, Chabi Fabrice Elegbede, Alexandra Papadopoulos, Albert Braeuning, Amélie Crépet, Alfonso Lampen,