کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8881035 | 1624815 | 2017 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Microwave-assisted extraction (MAE) conditions using polynomial design for improving antioxidant phytochemicals in Berberis asiatica Roxb. ex DC. leaves
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کلمات کلیدی
DADFRAPPBDDOEBBDAAEGAEDNA - DNA یا اسید دزوکسی ریبونوکلئیکdeoxyribonucleic acid - اسید deoxyribonucleicMAE - بلهOptimization - بهينه سازيPhenolic compounds - ترکیبات فنولیDiode-array detection - تشخیص دیود آرایهTAE - توResponse surface methodology - روش سطح پاسخRSM - روششناسی سطح پاسخCoefficient of Variation - ضریب تغییرdesign of experiment - طراحی آزمایشPlackett-burman design - طرح Plackett-BurmanBox-Behnken design - طرح جعبه BehnkenAntioxidant activity - فعالیت آنتیاکسیدانیMicrowave assisted extraction - مایکروویو کمک استخراجTotal tannins - مجموع تانن هاSEM - مدل معادلات ساختاری / میکروسکوپ الکترونی روبشیascorbic acid equivalent - معادل اسید اسکوربیکtannic acid equivalent - معادل اسید تاننGallic acid equivalent - معادل اسید گالیکquercetin equivalent - معادل کورستینScanning electron microscopy - میکروسکوپ الکترونی روبشیHimalaya - هیمالیاWatt - واتferric reducing antioxidant power - کاهش قدرت آنتی اکسیدان آهنHPLC - کروماتوگرافی مایعی کاراhigh-performance liquid chromatography - کروماتوگرافی مایعی کاراTotal flavonoids - کل فلاونوئیدهاTotal phenolics - کل فنولیکQuercetin - کوئرستینGallic acid - گالیک اسید
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Microwave-assisted extraction condition is of great importance for increasing recovery of phytochemicals with lesser time and solvent consumption. In this regard, the advanced microwave extraction technique was applied for the first time for improving polyphenolic antioxidants in Berberis asiatica leaves using polynomial design. A combination of first and second order polynomial designs was used at different levels using Plackett-Burman and Box-Behnken design and a total of 29 experiments were conducted for determination of optimal extraction condition for polyphenolics. Under optimal extraction condition [microwave power - 500Â W, sample to solvent ratio - 1:45 (g/ml) and methanol concentration - 60%], total phenolics (132.63Â mg GAE/g), total flavonoids (31.36Â mg QE/g), total tannins (145.17Â mg TAE/g), ABTS (34.46Â mM AAE/g), FRAP (708.07Â mM AAE/g) and DPPH (34.12Â mM AAE/g) antioxidant activities was recorded near close with predicted values. The extract also showed DNA and erythrocyte damage protective activity and reveals the presence of 12 phenolic compounds during high-performance liquid chromatography among which 4 compounds were reported first time from this species. Chlorogenic acid and catechin were found in higher concentration as compared with other phenolic compounds. The present study provides the potential of harnessing maximum antioxidant phytochemicals from the Berberis asiatica leaves using microwave-assisted extraction condition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Industrial Crops and Products - Volume 95, January 2017, Pages 393-403
Journal: Industrial Crops and Products - Volume 95, January 2017, Pages 393-403
نویسندگان
Tarun Belwal, Indra D. Bhatt, Ranbeer S. Rawal, Veena Pande,