کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2582111 | 1130222 | 2009 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cytotoxic aggregates of α-lactalbumin induced by unsaturated fatty acid induce apoptosis in tumor cells
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کلمات کلیدی
SOD1ESI-TOF MSFBSFITCMALDI-TOF8-anilino-1-naphthalenesulfonic acid3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide - 3- (4،5-dimethylthiazol-2-yl) -2،5-difenyltetrazolium bromideMTT - MTTα-La - α-لاα-Lactalbumin - α-لاکتبالومینStearic acid - اسید استئاریک Oleic acid - اسید اولئیکLinoleic acid - اسید لینولئیکFatty acid - اسید چربTem - این استAggregate - تجمیعApoptosis - خزان یاختهایcircular dichroism - رنگ تابی دورانیANS - سالfetal bovine serum - سرم جنین گاوCytotoxicity - سمیت سلولیsuperoxide dismutase 1 - سوپر اکسید دیسموتاز 1electrospray ionization time-of-flight mass spectrometry - طیف سنجی جرمی یونیزاسیون الکترو اسپری یونیزاسیونfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتMatrix assisted laser desorption/ionization time-of-flight - ماتریکس به مدت زمان پرواز از نظر جذب / یونیزاسیون لیزر کمک کردTransmission electron microscopy - میکروسکوپ الکترونی عبوریPropidium iodide - پروتئین یدیدMolten globule - گلوله گلوله
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The effects of three fatty acids on cytotoxic aggregate formation of Ca2+-depleted bovine α-lactalbumin (apo-BLA) have been studied by UV absorbance spectroscopy and transmission electron microscopy. The experimental results demonstrate that two unsaturated fatty acids, oleic acid and linoleic acid, and one saturated fatty acid, stearic acid, induce the intermediate of apo-BLA at pH 4.0-4.5 to form amorphous aggregates in time- and concentration-dependent manners. These aggregates are dissolved under physiological conditions at 37 °C and further characterized by fluorescence spectroscopy, circular dichroism and time-of-flight mass spectrometry. Our data here indicate that the structural characteristics of these aggregates are similar to those of HAMLET/BAMLET (human/bovine α-lactalbumin made lethal to tumor cells), a complex of the partially unfolded α-lactalbumin with oleic acid. Cell viability experiments indicate the aggregates of apo-BLA induced by oleic acid and linoleic acid show significant dose-dependent cytotoxicity to human lung tumor cells of A549 but those induced by stearic acid have no toxicity to tumor cells. Furthermore, the cytotoxic aggregates of apo-BLA induced by both unsaturated fatty acids induce apoptosis of human lung cancer cell line A549, suggesting that such cytotoxic aggregates of apo-BLA could be potential antitumor drugs. The present study provides insight into the mechanism of fatty acid-dependent oligomerization and cytotoxicity of α-lactalbumin, and will be helpful in the understanding of the molecular mechanism of HAMLET/BAMLET formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Chemico-Biological Interactions - Volume 180, Issue 2, 15 July 2009, Pages 131-142
Journal: Chemico-Biological Interactions - Volume 180, Issue 2, 15 July 2009, Pages 131-142
نویسندگان
Min Zhang, Fang Jr., Fang Yang, Jie Chen, Cong-Yi Zheng, Yi Liang,